Asparagus breslerianus a wild species in Iran, exhibited tolerance to salt in dry gypsum hills and dry lands. In order to check for salt tolerance thresholdvia in vitro conditions, the A. breslerianus callus was subjected to NaCl (sodium chloride) treatments. Six weeks old calli derived from male spear bud, were exposed to 0, 21.88, 43.76, 65.64, 76.58, 87.52, 109.40, 131.28, 153.16 and 175.04 mM NaCl in MS (Murashige and Skoog) basal medium supplemented with 0.88 µM BA (6-Benzylaminopurine) and 1.07 µM NAA (1- Naphthalene acetic acid). According to results, friable and compact type, green and white green calli were obtained up to 87.52 mM NaCl. Soft type, yellow and cream calli were observed with increasing salinity further 109.4 mM NaCl. Calli growth and plantlets regeneration were high in media up to 109.4 mM NaCl. In term of mineral accumulation, sodium content increased with an increase in NaCl levels. It indicates that A. breslerianus calli could acclimatize to salt stress by high osmotic adjustment. These suggested that, under in vitro salt-induced osmotic stress, A. breslerianus is reflected as salt resistant which tolerate NaCl up to 109.4 mM
