The Prevalence of Papillomavirus-16 and -18 isolated from women with cervical cancer using Multiplex PCR

Abstract

Background: Women infected with human papillomavirus, especially types 16 and 18, are at risk of cervical cancer. The purpose of this study was to determine the frequency of papillomavirus-16 and -18 in women with cervical cancer using multiplex-PCR. Materials and Methods: In this experimental study, after collecting blood samples, viral DNA was extracted using a Cinaclone kit, and PCR, with specific primers, was performed to detect HPV-16 and HPV-18. PCR products were analyzed by 1% agarose gel electrophoresis. Results: Of 60 samples, 19 were infected with HPV. The results showed that the frequency of genotype HPV-16 and HPV-18 was 8 (42.1%) and 11 (57.9%), respectively. Conclusion: The study showed that using PCR with specific primers for the detection of HPV-16 and HPV-18 is a convenient and accurate method. The results of this study indicate the relationship between HPV and cervical cancer