In
this study, a gas chromatography-tandem mass spectrometry (GC-MS/MS)
instrument, which has been widely used in recent years and has high separation
power, selectivity and ability to identify pesticides has been used. It is
aimed that the main criterion of this analytical method, in which the QuEChERS
methodology is used, is applicable to fast, easy, cheap, environmentally
friendly and different matrices. At the same time with this method, 123
pesticide residues and their degradation products were quantitatively assayed
by GC-MS/MS as well as method validations in tomatoe, lemon, lettuce, almonds,
raisins, honey, green pepper, milk and flour. Tomatoe was selected as potential
reference matrixes for the target. The steps of concentration and solvent
exchange were performed in the resultant extracts for the purpose of improving
analytical performance in terms of recovery, precision, linearity, of reducing
the amount of co-extracts. Multiple reaction monitoring (MRM) was used to
identify and quantify the pesticides. The samples were extracted with 1% acetic
acid in acetonitrile, anhydrous magnesium acetate, anhydrous magnesium sulfate
and clearing agent. For all pesticides, good linear calibrations with
coefficients (R2) ≥0.99 for nearly all of the analytes were obtained. Limit of quantitation of most of the
pesticides were in the range of 5-10 ng/g, and recovery of the method
validation accuracy parameter was done at two different concentrations 10 ng/g
and 50 ng/g were 88.6 - 99.7% and CV 1.60 – 14.0%