Determination of porcine oocyte and follicular fluid proteins from small, medium, and large follicles for cell biotechnology research

Abstract

Porcine oocytes from small, medium, and large follicles can be classified into five types. Type I (intact-cumulus oocytes) and type II oocytes (multi-cumulus cell layers surrounding oocytes) have high potential to be developed into in vitromatured oocytes. Determination of porcine follicular fluid (pFF) proteins from 3 follicle sizes by SDS-PAGE and LC/MS/MS, 6 protein bands sizes 52, 65, 79, 90, 160, and >220 kDa were identified as immunoglobulin gamma chain, keratin, porcine inhibitor of carbonic anhydrase, heat shock protein or plasminogen precursor or both, transthyretin, and protease. All proteins were reported for their important roles in promotion and regulation on growth and development of reproductive cells except for the protein band at 79 and 160 kDa which had diminished function in the first metaphase of oocyte. This study provides knowledge on the oocyte and pFF proteins as biological models and in vitro cell maturation supplements in biotechnology research