Markevych, Vitalii Eduardovych

Pohorielov, Maksym Volodymyrovych

Tarasova, Iryna Vitaliivna

Маркевич, Виталий Эдуардович

Маркевич, Віталій Едуардович

Погорелов, Максим Владимирович

Погорєлов, Максим Володимирович

Тарасова, Ирина Витальевна

Тарасова, Ірина Віталіївна

SocioEconomic Challenges Journal (Sumy State University)

LEAD TISSUE CONCENTRATION IN NEW BORN RATS DURING HYPOXIC LESSION 
Tarasova I. V., Markevich V. E., Pogorelov M. V. 
Sumy State University, pediatrics department with the course of medical genetics; 
department of hygiene and ecology with course of microbiology, virology and immunology 
 
Hypoxic-ischemic lesionsis one of importantproblems of neonatology, which is determined by theirplacein the 
structure ofmorbidity, perinatalmortality anda valuein the disordersformation. Microelements provide course of 
important biologicalreactions and are catalysts of manyof them.Micronutrient disbalanceisone of themechanisms 
ofdamage ofmembranes. The roleof microelements andespeciallyultramicroelementsin metabolicadaptation 
ofnewbornson the backgroundof hypoxiais staying unknown. Providing vital organs, such as cerebrum, heart, liver 
andkidneys, with microelements and ultramicroelements in the caseof hypoxia is uninvestigated too. Herewith,the 
roleof toxicmicroelements,namelylead, is not determined.  
The present objective was to research toxiclead dynamics in vital organstissues (brain, heart, liver, kidneys) of 
newborn rats in the case of experimentalhypoxiaof various severity degrees.  
All rats were randomly divided in two groups.  The first group (12 rats) was control. Hipobaric model of 
hypoxia was used in second experimental group (48 rats). Moderate hypoxia was achieved by the putting rates into 
hermetic chamber for 2 hours, where the air pressure of 525 millimeters of mercury column was created, which is 
corresponding to the partial pressure of oxygen of 110 millimeters of mercury column. The absorption ofcarbon 
dioxidein the chamber was exercised by soda lime. Hypoxia of the severe degree was achieved by the keeping animals 
for 2 hours in the chamber, where the air pressure was 380 millimeters of mercury column, which is corresponding to 
the partial pressure of oxygen of 80 millimeters of mercury column.  
The animals were taken out of the experiment after 12 hours and on the 7
th
 day. Brain, heart, liver and kidneys 
were removed aseptically and weighed. Organs were dried for 36 h and digested with a mixed solution of hydrochloric 
and nitric acids (1:3, vol/vol) and slowly heated to 100
0
 C until the digestion was complete and added by 10ml by 
distilleted watter.Brain, heart, liver and kidneys were removed and measure of  Pb in this organs.  
The content oftoxicleadis the largestin brainand almosttwiceincreases in liver, heart andkidneys.The levelof 
leadis stableduring the firstweek of lifein liverandheart, butits content inkidneysincreasesin three times as much as 
decrease in brain tissues of rats. Effect ofmoderatehypoxialeadsto increase ofaccumulationof leadin heartand kidneys– 
in 8 times, in liver– in 3 timesandin brain(28.2%, p <0.05). In case of severe hypoxia we can see a decreaseof lead 
contentin liver, but in other organsits content ismuchhigher thanresults of control groupandanimals,affected 
bymoderatehypoxia. Accumulationof leadis accompanied byformationof correlations of medium strengthin 
kidneysandliver(r = 0,43)under conditionsof moderatehypoxiaandthe strongconnection- in case ofseverehypoxicinjury(r 
= 0,76).A significantpowerrelationshipis formedabout theelementcontentin heartand kidneysbothin the caseof light(r = 
0,92),andsevere(r = 0,81)hypoxia. Two-factor analysis of variance of influence of controlled factors on the content of 
microelements shows significant influence of age and hypoxia. The age factor has predominant influence in brain 
tissues, heart and kidneys. In liver lead content is equal dependent on age and degree of hypoxic damage. 
 


English

Lead tissue concentration in new born rats during hypoxic lession

Electronic Sumy State University Institutional Repository

LEAD TISSUE CONCENTRATION IN NEW BORN RATS DURING HYPOXIC LESSION Tarasova I. V., Markevich V. E., Pogorelov M. V. Sumy State University, pediatrics department with the course of medical genetics; department of hygiene and ecology with course of microbiology, virology and immunology  Hypoxic-ischemic lesionsis one of importantproblems of neonatology, which is determined by theirplacein the structure ofmorbidity, perinatalmortality anda valuein the disordersformation. Microelements provide course of important biologicalreactions and are catalysts of manyof them.Micronutrient disbalanceisone of themechanisms ofdamage ofmembranes. The roleof microelements andespeciallyultramicroelementsin metabolicadaptation ofnewbornson the backgroundof hypoxiais staying unknown. Providing vital organs, such as cerebrum, heart, liver andkidneys, with microelements and ultramicroelements in the caseof hypoxia is uninvestigated too. Herewith,the roleof toxicmicroelements,namelylead, is not determined.  The present objective was to research toxiclead dynamics in vital organstissues (brain, heart, liver, kidneys) of newborn rats in the case of experimentalhypoxiaof various severity degrees.  All rats were randomly divided in two groups.  The first group (12 rats) was control. Hipobaric model of hypoxia was used in second experimental group (48 rats). Moderate hypoxia was achieved by the putting rates into hermetic chamber for 2 hours, where the air pressure of 525 millimeters of mercury column was created, which is corresponding to the partial pressure of oxygen of 110 millimeters of mercury column. The absorption ofcarbon dioxidein the chamber was exercised by soda lime. Hypoxia of the severe degree was achieved by the keeping animals for 2 hours in the chamber, where the air pressure was 380 millimeters of mercury column, which is corresponding to the partial pressure of oxygen of 80 millimeters of mercury column.  The animals were taken out of the experiment after 12 hours and on the 7th day. Brain, heart, liver and kidneys were removed aseptically and weighed. Organs were dried for 36 h and digested with a mixed solution of hydrochloric and nitric acids (1:3, vol/vol) and slowly heated to 1000 C until the digestion was complete and added by 10ml by distilleted watter.Brain, heart, liver and kidneys were removed and measure of  Pb in this organs.  The content oftoxicleadis the largestin brainand almosttwiceincreases in liver, heart andkidneys.The levelof leadis stableduring the firstweek of lifein liverandheart, butits content inkidneysincreasesin three times as much as decrease in brain tissues of rats. Effect ofmoderatehypoxialeadsto increase ofaccumulationof leadin heartand kidneys– in 8 times, in liver– in 3 timesandin brain(28.2%, p <0.05). In case of severe hypoxia we can see a decreaseof lead contentin liver, but in other organsits content ismuchhigher thanresults of control groupandanimals,affected bymoderatehypoxia. Accumulationof leadis accompanied byformationof correlations of medium strengthin kidneysandliver(r = 0,43)under conditionsof moderatehypoxiaandthe strongconnection- in case ofseverehypoxicinjury(r = 0,76).A significantpowerrelationshipis formedabout theelementcontentin heartand kidneysbothin the caseof light(r = 0,92),andsevere(r = 0,81)hypoxia. Two-factor analysis of variance of influence of controlled factors on the content of microelements shows significant influence of age and hypoxia. The age factor has predominant influence in brain tissues, heart and kidneys. In liver lead content is equal dependent on age and degree of hypoxic damage.  

https://essuir.sumdu.edu.ua/bitstream/123456789/27506/1/Tarasova.pdf

Lead tissue concentration in new born rats during hypoxic lession

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SocioEconomic Challenges Journal (Sumy State University)

Electronic Sumy State University Institutional Repository