Condensation of benzil (1) with urea, monophenyl urea and diphenyl urea in the presence of absolute ethanol using 30% aqueous NaOH gave the products 1a, 1b and 1c respectively and also with thiourea, monomethyl thiourea, dimethyl thiourea and diethyl thiourea the products 2a, 2b, 2c and 2d were obtained. Methylation of the product, 2a in the presence of dimethyl formamide (DMF) using K2CO3 formed 2. The compounds 1b, 1c, 2b, 2c and 2 showed highly cytotoxic activity and the compounds 1a, 2a, 2d showed relatively low cytotoxic activity against brine shrimp lethality bioassay

A. Kashem Liton

M. Rabiul Islam

Bangladesh Journal of Pharmacology

A Kashem Liton

M Rabiul Islam

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Synthesis of hydantoin and thiohydantoin related compounds from benzil and study of their cytotoxicity

Introduction Several types of substituted isatin heterocyclic deriva-tives were synthesized and found cytotoxic activity of these compounds by screening tests (Islam et al., 2001a; Islam et al., 2001b; Lingcon et al., 2001). Methyl and bro-mine groups in the benzene ring of isatin, Δ2-1,3,5-thiadiaozolines show more cytotoxic activity. For this interest, the title compounds have been synthesized for screening tests whether they show reasonable lethal effect on brine shrimp or not. We report to see herein the results of the synthesis of the mentioned com-pounds (Scheme 1), spectral characterization and their cytotoxic effects by brine shrimp lethality bioassay (Anderson et al., 1999).  Materials and Methods Melting points are not corrected. IR spectra recorded on a Shimadzu DR 8001 FT-IR spectrometer, NMR spectra on a WP 200 spectrometer using TMS as internal standard and mass spectra on an MS Kratas mass spec-trometer.  Preparation of mono- and diphenyl urea Mono-phenyl urea and diphenyl urea were prepared from aniline hydrochloride and urea following the reported procedure (Furniss et al., 1998).  Preparation of 5,5-diphenylhydantoin (1a) (Muccioli et al., 2003) Benzil, 1 (0.8 g, 3.8 mmol/L) was placed in a 100 mL round-bottomed flask with urea, (0.4 g, 6.7 mmol/L) in the molar ratio of ca 1:2. Absolute ethanol (12 mL) and 30% aqueous sodium hydroxide (2.5 mL) were added to these reactants. Boiling chips were also added to this solution and a condenser was attached after wrapping the ground glass joint with Teflon tape, and was heated (110-120°C) the mixture under reflux (2 hours). The reaction mixture was cooled before adding 15 mL of water. The solution was not clear, so the suspended solids were removed by filtration. Then the clear solu-A Journal of the Bangladesh Pharmacological Society (BDPS)                                        Bangladesh J Pharmacol 2006; 1: 10-15Journal homepage: www.banglajol.infoAbstracted/indexed in Academic Search Complete, Asia Journals Online, Bangladesh Journals Online, Biological Abstracts, BIOSIS Previews, CAB Abstracts, Current Abstracts, Directory of Open Access Journals, EMBASE/Excerpta Medica, Global Health, Google Scholar, HINARI (WHO), International Pharmaceutical Abstracts, Open J-gate, Science Citation Index Expanded, SCOPUS and Social Sciences Citation Index; ISSN: 1991-0088AbstractCondensation of benzil (1) with urea, monophenyl urea and diphenyl urea in the presence of absolute ethanol using 30% aqueous NaOH gave the products 1a, 1b and 1c respectively and also with thiourea, monomethyl thiourea, dimethyl thiourea and diethyl thiourea the products 2a, 2b, 2c and 2d were obtained. Methylation of the product, 2a in the presence of dimethyl formamide (DMF) using K2CO3  formed 2. The compounds 1b, 1c, 2b, 2c and 2 showed highly cytotoxic activity and the compounds 1a, 2a, 2d showed relatively low cytotoxic activity against brine shrimp lethality bioassay.  Article InfoReceived:  15 December 2005 Accepted:  20 January 2006 Available Online:   3 January 2008 DOI: 10.3329/bjp.v1i1.481 Cite this article: Liton AK, Islam MR. Synthesis of hydantoin and thiohydantoin related compounds from benzil and study of their cytotoxicity. Bangladesh J Phar-macol. 2006; 1: 10-15. Synthesis of hydantoin and thiohydantoin related compounds from benzil and study of their cytotoxicity  A. Kashem Liton and M. Rabiul IslamDepartment of Chemistry, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.This work is licensed under a Creative Commons Attribution 4.0 License. You are free to copy, distribute and perform the work. You must attribute the work in the manner specified by the author or licensor.  tion was cautiously acidified with concentrated hydro-chloric acid and the product was collected by vacuum filtration and washed thoroughly with water.  Then the product, 1a was recrystallized by ethanol, dried, the m.p. 294-295ºC and yields 0.750 g (80%). IR: ν Nujol (cm-1) 3271 (s, νΝ-H amide, CO-NH-CO); 3204 (s,νΝ-H amide, CO-NH-CPh2); 3055 (m, νC-H aromatic);1772 (s, νC=O amide, NH-CO-CPh2); 1741 (s, νC=Oamide, NH-CO-NH); 1597, 1541, 1508 (s, νC=C,aromatic). 1H-NMR (DMSO): δ 10.10 (s, 1H, NH, CO-NH-CO); δ 9.10 (s, 1H, NH, CO-NH-CPh2); δ 7.80-7.00(m, 10H, C-H, aromatic). 13C-NMR (DMSO): δ 175.19 (C-4); δ 156.36 (C-2); δ 140.27 (C-6); δ 128.87 (C-7); δ 128.39(C-8); δ 126.93 (C-9); δ 70.56 (C-5). Mass: m/z (% ofrelative intensity) 252 (M+.3%), 176 (30%), 154 (100%),128 (3%), 77 (15%), 51 (8%), and 39 (10%). The molecularion peak appears at m/z 252 due to C15H12N2O2.Preparation of 3,5,5-triphenyl hydantoin (1b) Benzil, 1; (0.148 g, 0.70 mmol/L) and the monophenyl-urea; (0.298 g, 1.40 mmol/L) in the molar ratio of ca 1:2 were refluxed in the absolute ethanol and the procedure for 1b was similar to that of the compound, 1a. The product, 1-a1 was colorless solid; m.p. 172-173ºC having Bangladesh J Pharmacol 2006; 1: 10-15  11 Scheme 1  yields 0.077 g, 50%. IR: ν Nujol (cm-1) 3220 (s, νΝ-H amide); 3055 (m, νC-H aromatic); 1716 (s, νC=O amide, NPh-CO-C-Ph2); 1637 (s, νC=O amide, NH-CO-NPh); 1595, 1541, 1508 (νC=C, aromatic). 1H-NMR (DMSO): δ 9.40 (s, 1H, NH, amide); δ 7.80-7.40 (m, 15H, C-H, aromatic). 13C-NMR (DMSO): δ 173.06 (C-4); δ 154.32 (C-2); δ 141.99 (C-10); δ 140.60 (C-6); δ 129.05 (C-7) δ 128.30 (C-8); δ 127.97 (C-9); δ 127.33 (C-12); δ 121.53 (C-13); δ 117.70 (C-11); 68.70 (C-5). Mass: m/z (%of relative intensity) 328 (M+.3%), 176 (25%), 154 (100%), 136 (68%), 120 (12%), 55 (27%), 43 (26%). The molecular ion peak appears at m/z 328 due to C21H16N2O2.  Preparation of 1,3,5,5-Tetraphenyl hydantoin (1c) Benzil 1; (0.148 g, 0.70 mmol/L) and the above 1,3-diphenylurea, a3; (0.298 g, 1.40 mmol/L) in the molar ratio of ca 1:2 were refluxed in the absolute ethanol and the procedure of 1c was similar to that of the compound, 1a. The product, 1c was off white powder. It was recrystallized in methanol, dried and m.p. 163-165ºC and yields were 0.07 g, 42%. IR: ν Nujol (cm-1) 3110 (w, νC-H aromatic); 1716 (s, νC=O amide, NPh-CO-CPh2); 1635 (s, νC=O amide, NPh-CO-NPh); 1595, 1541, 1508 (νC=C, aromatic). 1H-NMR (DMSO): δ 7.20-6.70 (m, 20H, C-H, aromatic). 13C-NMR (DMSO): δ 173.10 (C-4); δ 154.25 (C-2); δ 141.88 (C-10); δ 140.60 (C-6); δ 139.88 (C-14) δ 129.33 (C-7) δ 128.71 (C-8); δ 127.99 (C-9); δ 127.38 (C-12); δ 127.26 (C-13); δ 127.12 (C-16) δ 121.60 (C-17); δ 117.73 (C-11); δ 109.48 (C-15) δ 69.50 (C-5). Mass: m/z (%of relative intensity) 404 (M+.3%), 369 (75%), 347(68%), 302 (15%), 259 (7%), 233 (12%), 211 (100%), 182 (92%), 136 (29%), 93 (41%). The molecular ion peak appears at m/z 404 due to C27H20N2O2.  Preparation of 5,5-diphenyl-2-thiohydantoin (2a) The compound, 2a was prepared from Benzil, 1; (0.80 g, 3.81 mmol/L) and thiourea; (0.58 g, 7.63 mmol/L) following the procedure of 1a. The product was recrystallized in ethanol, m.p. 234-235ºC yielded 0.595 g, 94%. IR: ν Nujol (cm-1) 3255 (s, b, νN-H amide, CO-NH-CS); 3135 (b, νN-H, amide, CPh2-NH-CS); 3010 (s, C-H aromatic); 1749 (s, νC=O amide); 1558, 1541, 1508 (νC=C, benzene); 1215 (s, νC=S). 1H-NMR (DMSO): δ 7.80-7.60 (m, 10H, C-H, aromatic), δ 1.10-1.12 (1H, SH). 13C-NMR (DMSO): δ 181.65 (C-4); δ 175.54 (C-2); δ 138.70 (C-6); δ 129.13 (C-7); δ 128.80 (C-8); δ 126.92 (C-9); 73.92 (C-5). Mass: m/z (% of relative intensity) 268 (M+.12%), 182 (18%), 154 (100%), 136 (59%), 120 (12%), 107 (19%), 77 (17%), 57 (27%), 43 (23%). The molecular ion peak appears at m/z 268 due to C15H12N2OS.  Preparation of 3-methyl-5, 5-diphenyl-2-thiohydantoin (2b) Benzil, 1; (0.84 g, 4.00 mmol/L) with methylthiourea (0.72 g, 8.00 mmol/L) in the molar ratio of ca 1:2 were refluxed in ethanol and the procedure for the compound, 2b is similar to that of the compound, 1a. The product was recrystallized in ethanol; m.p. 182-183ºC having yields 1.0 g, 94%. IR: ν Nujol (cm-1) 3250 (b, νN-H thiamide); 3110 (s, w, νC-H aromatic); 2950, 2900 (s, νC-H, aliphatic), 1716 (s, νC=O amide); 1595, 1541, 1508 (νC=C, aromatic); 1215 (s, νC=S). 1H-NMR (DMSO): δ 7.20-6.70 (m, 10H, C-H, aromatic), δ 3.50 (s, 3H, C-H, aliphatic), δ 1.30 (s, 1H, SH). 13C-NMR (DMSO): δ 181.94 (C-2); δ 173.93 (C-4); δ 138.55 (C-6); δ 129.13 (C-7) δ 128.89 (C-8); δ 127.44 (C-9); δ 71.76 (C-5); δ 27.79 (C-10). Mass: m/z (%of relative intensity) 282 (M+.100%), 205 (22%), 180(68%), 165 (50%), 121 (7%), 104 (72%), 77 (49%), 59 (6%), 51 (17%), 39 (3%). The molecular ion peak appears at m/z 282 due to C16H14N2OS.  Preparation of 1,3-dimethyl-5, 5-diphenyl-2-thiohy-dantoin (2c) Compound, 2c was obtained from benzil, 1; (1.00 g, 4.76 mmol/L) and 1,3-dimethylthiourea; (1.00 g, 9.61 mmol/L) in ethanol following the procedure of 1a. Com-pound 2c was obtained as white crystalline solids, m.p. 154-156ºC having 0.950 g, yielded 85%. IR: ν Nujol (cm-1) 3030 (s, νC-H aromatic); 2930, 2858 (s, νC-H, aliphatic); 1643 (s, νC=O amide); 1470, 1448 (s, νC=C, aromatic); 1296 (s, νC-N); 1126 (s, νC=S stretching vibration). 1H-NMR (DMSO): δ 7.00-6.60 (m, 10H, C-H, aromatic), δ 3.30 (bs, 6H, CH3). 13C-NMR (DMSO): δ 184.08 (C-2, C-4); δ 137.90 (C-6); δ 127.97 (C-7) δ 127.74 (C-8); δ 127.12 (C-9); δ 95.39 (C-5); δ 33.41 (C-10); δ 30.54 (C-11). Mass: m/z (%of relative intensity) 296 (M+. 100%), 224 (23%), 209(13%), 176 (10%), 154 (31%), 136 (45%), 105 (56%), 91 (13%), 77 (14%), 43 (12%). The molecular ion peak appears at m/z 296 due to C17H16N2OS.  Preparation of 1,3-diethyl-5, 5-diphenyl-2-thiohydan-toin (2d) Refluxing of benzil, 1; (0.84 g, 4.00 mmol/L) and 1,3-diethylthiourea; (1.06 g, 8.00 mmol/L) and the follow-ing procedure of 1a gave the product 2d. The product was recrystallized in ethanol, m.p.116-117ºC having yielded 0.297 g, 45%. IR: ν Nujol (cm-1) 3110 (w, νC-H aromatic); 2990, 2830 (s, νC-H, aliphatic); 1716 (s, νC=O amide, NPh-CO-C-Ph2); 1635 (s, νC=O amide, NPh-CO-NPh); 1595, 1541, 1508(νC=C, aromatic).  12 Bangladesh J Pharmacol 2006; 1: 10-15                                                                 1H-NMR (DMSO): δ 7.00-6.80 (m, 10H, C-H, aromatic), δ 3.60 (q, 2H, 11CH2), δ 3.30 (q, 2H, 13CH2), δ 1.20 (t, 6H, CH3). 13C-NMR (DMSO): δ 183.31 (C-2); δ 183.21 (C-4); δ 140.07 (C-6); δ 128.17 (C-7); δ 127.97 (C-8); δ 127.83 (C-9); δ 99.62 (C-5); δ 96.54 (C-11); δ 96.28(C-13); δ 14.85 (C-12); δ 14.43 (C-14). Mass: m/z (%of relative intensity) 324 (M+.12%), 194 (28%), 165(19%), 150 (55%), 121 (12%), 105 (100%), 86 (9%), 77 (50%), 51 (5%), 29 (21%). The molecular ion peak appears at m/z 324 due to C19H20N2OS.  Preparation of S-methyl-5, 5-Diphenyl-2-thiohydan-toin (2) (Muccioli et al., 2003) The compound, 2a (0.200 g, 0.71 mmol/L) was dissolved in anhydrous DMF (0.70 mL) and K2CO3 (0.41 g, 0.31 mmol/L) then iodomethane (0.70 mL, 0.71 mmol/L) were added to this mixture and stirred overnight at room temperature. The mixture was poured into distilled water. The resulting precipitate was collected, dried and recrystallized from ethanol (If some of K2CO3 are non-reacted then hot ethanol solution of product is filtered to separate K2CO3 from the product) the product, 2. The yields of the product, 2 were 0.75 g, 38% having m.p.172–174ºC. IR: ν Nujol (cm-1) 3240 (w, νN-H, amine); 3010 (s, νC-H, aromatic); 2900, 2855 (s, νC-H, aliphatic); 1725 (s, νC=O); 1684 (s, νC=N); 1585, 1506, 1489 (νC=C, aromatic). 1H-NMR (DMSO): δ 7.40-7.20 (m, 10H, C-H, aromatic), δ 3.20 (s, 1H, NH), δ 2.70 (s, 3H, C-H, aliphatic). 13C-NMR (DMSO): δ 180.36 (C-2); δ 162.42 (C-4); δ 140.80 (C-6); δ 128.72 (C-7); δ 127.97 (C-8); δ 127.14 (C-9); δ 77.94 (C-5); δ 26.87 (C-10). Mass: m/z (%of relative intensity) 282 (M+.2%) 176 (27%), 165(12%), 154 (100%), 136 (70%), 120 (13%), 107 (19%), 88 (18%), 77 (15%), 51 (5%), 43 (8%). Scheme 2  Bangladesh J Pharmacol 2006; 1: 10-15                                                                 13  The molecular ion peak appears at m/z 282 due to C16H14N2OS.    Results and Discussion Simple base catalyzed condensation of benzil, 1 with urea, monophenyl urea and diphenyl urea in absolute ethanol furnished 1a, 1b, 1c whereas with thiourea, methyl thiourea, dimethyl thiourea and diethyl thiourea gave 2a, 2b, 2c and 2d. The formation of these products follows the pinacole-pinacolone type (Shukla and Trivedi, 1997) rearrangement that is shown in the Scheme 2 (e.g., compound, 1a). The IR spectrum of the compound, 1a at 3271 cm-1 indicates the presence of NH group of amide moiety. The other band at 3204 cm-1 also indicates the presence of NH in Ph2C-NH-CO. The clear band at 3055 cm-1 shows the presence of C-H stretching vibration of phenyl ring. The lower absorption at 1772 cm-1 assigns C=O group of amide in Ph2-CO-NH-, on the other hand the band at 1741 cm-1 corresponds to other C=O group in NH-CO-NH. The bands at 1597 cm-1, 1541 cm-1, 1508 cm-1 give the strong support of C=C stretching vibration of phenyl ring. 1H-NMR spectrum shows two singlets at δ 10.1 and δ 9.1, which would be assigned for NH proton of amide. The aromatic protons appear as multiplet at δ 7.8-7.0. 13C-NMR spectrum at δ 175.2 (C-4) and δ 156.4 (C-2) is for the carbonyl groups of amide. The aromatic carbons are designated by the following values δ 140.3 (C-6), δ 128.9 (C-7), δ 128.4 (C-8), δ 126.9 (C-9) and at δ 70.6 (C-5). In the MS spectrum of the compound, 1a the molecular ion peak (M+.3%) appears at m/z 252 that corresponding to the molecular formula C15H12N2O2. In this spectrum the base peak is formed at m/z 154.  Methylation of 2a with CH3I in the presence of K2CO3 in dry DMF gave the product (2) In the IR spectrum of the compound, 2 the sharp band at 3240 cm-1 indicates the presence of NH group of amide. The signal at 3010 cm-1 locates the presence of C-H stretching vibration in aromatic. The bands at 2900 cm-1 and 2855 cm-1 indicate the presence of C-H group for CH3. The clear and sharp band at 1725 cm-1 is assigned for C=O group. The band at 1684 cm-1 indicates the presence of C=N group. The bands at 1585, 1506 and 1489 give the strong support for the presence of C=C stretching vibration in the aromatic ring. In 1H-NMR spectrum the aromatic protons appear as multiplet at δ 7.4-7.2. The singlet at δ 3.2 indicates the presence of NH group and the singlet at δ 2.7 is observed for CH3 group in S-CH3. In 13C-NMR spectrum the signals at δ 180.4 (C-2) and δ 162.4 (C-4) appears for both C=S and C=O groups respectively. The aromatic carbons are designated by the following values) δ 140.8 (C-6), δ 128.7 (C-7), δ 128.0 (C-8), δ 127.1 (C-9). The value at 77.9 (C-5) appears for the aliphatic carbon with various substituted groups. The signal at δ 26.9 (C-10) is concerned for simple aliphatic carbon CH3. In the MS spectrum the molecular ion peak (M+.2%) appears at m/z 282 that corresponding to the molecular formula C16H14-N2OS. In this spectrum the base peak is formed at m/z 154.  Cytotoxicity Cytotoxicity of all the compounds was measured by brine shrimp lethality bioassay method. Cisplatin, a recognized anti-cancer drug was used as reference to compare the efficacy of the synthesized compounds. Compounds 1a, 1b, 1c, 2a, 2b, 2c, 2d and 2 showed potential cytotoxicity but compounds 1b, 1c, 2b, 2c and 2 that showed highly cytotoxic activity and compounds 1a, 2a, 2d showed relatively low cytotoxic activity. The LC50 values of the titled compounds are represented in the Figure 1. 1.07 1.05 0.95 1.25 1.05 1.03 1.1 1.03 1.4  1.2  1  0.8  0.6  0.4  0.2  0 LC50 1a                     1b                     1c                     2a                     2b                     2c                    2d                     2 Sample number Figure 1: Comparative graphical representation of the LC50 of toxicity for the synthesized compounds against brine shrimp lethali-ty test  14 Bangladesh J Pharmacol 2006; 1: 10-15                                                                 Author Info A. Kashe Liton (Principal contact) e-mail: litonchem@yahoo.com   Financial Support Self-funded  References Anderson JE, Goetz CM, McLaughlin JL, Suffness M. Phyto-chemical analysis. Oxford University Press, 4th ed. 1991, p 107.  Furniss BS, Hannaford AJ, Rogers V, Smith PWG, Tatehell AR. Vogel’s Textbook of practical organic chemistry. Longman Press, 4th ed., 1998, pp 734-35.  Islam MR, Khayer K, Mahmud MI. Reaction of isatin with 2-aminothiophenol leading to spiroheterocyclic having anti-cancer activity. Jahangirnagar Univ J Sci. 2001a; 24: 17-22.  Islam R, Khayer K, Abedin MJ, Islam MR. Synthesis of (5-spiro(5´-methylisat in) -4-acetyl -2-(acetylamino)Δ2 -1,3 ,4 -thiadiazoline and 5-spiro (5´-methylisatin)-4-acetyl-2-(5´-methylisatin-3´-hydrazineo)-Δ2-1,3,4-thiadiazoline. Indian J Chem. 2001b; 40B: 240-42.  Lingcon MH, Islam R, Khayer K, Islam MR. Cyclization of substituted indole-2-one-3-thiosemicarbazones to noble heterocyclic systems. J Bangladesh Chem Soc. 2001; 14: 127-32.  Muccioli GG, Poupaert JH, Wouters J, Norberg B, Poppitz W, Scriba GKE, Lambert DM. A rapid and efficient microwave-assisted synthesis of hydantoins and thiohydantoins. Tetra-hedron 2003; 59: 1301-07.  Shukla SP, Trivedi GL. Modern organic chemistry. 1st ed. S. Chand and Company Ltd., 1997, p 40.   Bangladesh J Pharmacol 2006; 1: 10-15                                                                 15 

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Synthesis of hydantoin and thiohydantoin related compounds from benzil and study of their cytotoxicity

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