本研究的目的為使用不同種動物不同糞
脂醇類分子化合物之含量與比例的特性，作
為台灣本土人源與動物源污廢水污染河川水
體之標記的可行性研究。糞脂醇是高等哺乳
類腸道分解排出之特異性物質，其於環境中
與底泥沉澱微粒結合後非常穩定，故可以之
作為生物源污染之依據。樣本首先以二氯甲
烷萃取並以MSTFA 及催化劑（ TMSI /
dichloromethane / triethylamine, 142:858:2）衍
生，再以氣相層析質譜儀定性與定量分析脂
醇類化合物。本土樣本特性則以市鎮污水與
畜牧廢水（豬源與牛源）脂醇類之量與種類
或比例之關係，將之歸納比較後，求得區分
指標值即（ Coprostanol ÷ Epicoprostanol ） ×
Coprostanone，因不同源有不同之適用標記，
故可做為環境中各類生物污染監測依據。廢
水處理前後脂醇類之種類與量之不同，亦可
為畜牧廢水有否處理與適切處理後排放檢測
根據之用。The aim of this research was to study the
possibility of utilizing animal fecal sterols
from humans and animals as the markers to
distinguish fecal pollution in receiving waters
of Taiwan’s river. Fecal sterols are present in
mammalian feces and they are reduced from
cholesterol by the colonic microbacteria.
Sterols are stable in the environment when they
are absorpted to particulate matters in the
sediment and sludge. Thus, after clean up and
extraction, sterols can be qualitated and
quantitated by gas chromatography/mass
spectrometric analysis. These sterols can be the
good indicators of biopollution markers.
Optimization of the cleanup, extraction, and
GC/MS analysis are studied in this study.
Domestic sewage, livestock wastewaters were
investigated thereafter to characterize the
specific types, amount and their ratio of the
sterols, and these spectra were sreved as the
field monitoring reference indicators whether
livestock wastewater are treated or not before
they are dumped into the receiving waters
