Polymer-coated superparamagnetic iron oxide nanoparticles as T2 contrast agent for MRI and their uptake in liver

Abstract

[Aim]: To study the efficiency of multifunctional polymer-based superparamagnetic iron oxide nanoparticles (bioferrofluids) as a T2 magnetic resonance contrast agent and their uptake and toxicity in liver. [Materials & methods]: Mice were intravenously injected with bioferrofluids and Endorem®. The magnetic resonance efficiency, uptake and in vivo toxicity were investigated by means of magnetic resonance imaging (MRI) and histological techniques. [Results]: Bioferrofluids are a good T2 contrast agent with a higher r2/r1 ratio than Endorem. Bioferrofluids have a shorter blood circulation time and persist in liver for longer time period compared with Endorem. Both bioferrofluids and Endorem do not generate any noticeable histological lesions in liver over a period of 60 days post-injection. [Conclusion]: Our bioferrofluids are powerful diagnostic tool without any observed toxicity over a period of 60 days post-injection.Financial support from the Spanish Ministry of Science and Innovation research grant MAT2014-54975-R, and from the Programa Operativo FEDER Aragon 2014-2020 ‘Construyendo Europa desde Aragon’, is gratefully acknowledged. Additional support from the Diputacion General de Aragon (DGA-M4) is also acknowledged. LMA Ali acknowledges financial support from the Spanish Ministry of Science and Innovation FPI research grants. The Servicio de Experimentacion Animal (SAI), and servicio de Microscopia Electronica de Materiales of Zaragoza University. P Marzola acknowledges financial support from Fondazione Cariverona (Verona,Italy) through the project ‘Verona Nanomedicine Initiative’ and from MIUR through FIRB project RBAP114AMK – RI.NA.ME. ‘Rete Integrata per la Nanomedicina’.Peer reviewe