Asselman, J.

De Schamphelaere, K.

Janssen, C.R.

Mees, J.

Semmouri, I.

Open Marine Archive

138 
Poster presentation Competition poster 
Metatranscriptome of a marine pelagic crustacean community using 
nanopore sequencing  
Semmouri Ilias1, De Schamphelaere Karel1, Mees Jan2, Janssen Colin1 and Asselman Jana1 
1  Universiteit Gent, Onderzoeksgroep voor Milieutoxicologie (UGent-ECOTOX), Coupure Links 
653, 9000 Gent, Belgium  
E-mail: ilias.semmouri@ugent.be  
2  Flanders Marine Institute (VLIZ), InnovOcean site, Wandelaarkaai 7, 8400 Oostende, Belgium 
 
Due to their rapid responses to environmental variation, planktonic organisms are used as bio-
indicators of ecosystem changes. With the need for better understanding the impact of a 
changing environment on zooplankton communities, zooplankton monitoring programs have 
been carried out in the marine environment globally since the early 20th century. Most 
zooplankton monitoring studies focus mainly on variability in biodiversity and biomass. 
However, this approach is hindered by challenges in the identification, which is time-consuming, 
complicated and requires biological expertise. Advances in practical, cost-effective molecular 
approaches, such as (meta)barcoding, helped overcome the issues with morphology-based 
biomonitoring. Yet, a more comprehensive molecular data set would be able to identify and 
assess the impact of the main drivers of changes in the marine ecosystem, rather than only 
determining species richness. Studying the functional activities of a community has been 
facilitated by metatranscriptomics, the study of community gene transcription. 
Metatranscriptomics is generally employed to assess both the functional and the taxonomic 
components of a community. Here, we sequenced the metatranscriptome of a pelagic 
crustacean community in the BPNS, using the MinION™, a portable nanopore-based DNA/RNA 
sequencing platform (Oxford Nanopore Technologies). We establish a method for capturing the 
metatranscriptome of zooplankton communities in marine samples. 
 
Keywords: Zooplankton; Copepods; Metatranscriptome; Nanopore sequencing; MinIon 
  


Metatranscriptome of a marine pelagic crustacean community using nanopore sequencing

http://www.vliz.be/imisdocs/publications/325011.pdf

Metatranscriptome of a marine pelagic crustacean community using nanopore sequencing

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