Does pore scale biogeography exist in different soil types?

Abstract

Microbial activity is largely controlled by the abiotic conditions prevailing in their habitats, which are veryheterogeneous at the microscopic scale. A few studies have demonstrated a microbial biogeography at thepore scale. Different regions in the soil pore network can be considered to be different microbial habitats andthese different regions have been shown to be well correlated with organic carbon mineralization rates. Weaimed to test whether such a functional biogeography exists for different soil types and whether it is consistentacross soils. We selected six topsoils with contrasted texture, soil organic matter content and pH (2 cambisols,3 luvisols and 1 podzol under different managements). We added a 13C labelled, easily mineralisable organicsubstrate, pyruvate, to soil samples previously equilibrated at different matric potentials, in order to place thesubstrate preferentially in soil pores with neck diameters of 3 to 10 μm or 30 to 10μm, according to the Jurin-Laplace law. The soil samples were then incubated at pF 1.5 for 3 weeks and CO2 and 13C-CO2 weremonitored. At the end of incubation, total and 13C-PLFA were extracted and analysed. Basal mineralisation,expressed as % total organic C was affected by soil type, mainly related soil pH and the quality[NN1] of theorganic matter. The 6 soils exhibited contrasted microbial community composition, as shown by their PLFAprofiles. The addition of pyruvate did not induce any priming effect in soils, except in the long term bare fallowsoil, where the mineralization of SOM was presumably limited by energy. In the long term bare fallow soil,pyruvate mineralization was the same whatever the region it was placed in, suggesting other controls of itsmineralization than the characteristics of pore scale habitats. In four soils out of six, the mineralization ofpyruvate was more rapid when it was initially placed in large pores (30 to 100μm) than in small pores (3 to 10μm), suggesting that pore scale biogeography may be a general feature in soils and that coarser pores are morefavourable habitats for soil organic matter mineralization