Fakultas Matematika dan Ilmu Pengetahuan Alam UNHAS
Abstract
Molecular identification of bacterial simbiont macroalgae sargassum polycystum producing enzymes L-asparaginase were isolated from Barrang Lompo Island South Sulawesi in 2013. Among them three bacterial strains exhibiting glutaminase free L-asparaginase activity were identified as pseudomonas putida, enterobacter asburine and marinobacter arcticus on the basis of 16S rRNA gene sequencing. The Highest max score 1358 and query cover 98 % were pseudomonas putida furthermore identified as bacterial L-asparaginase activity from sargassum sp. Pseudomonas putida has Identities 756/765 (99%) with 778 bp Query length, Query cover 98% and Gaps 5/765 (0%)
