Kinetic characterization of MenD enzyme in the reaction of bicatalytical synthesis of 6-cyano-4-oxohexanoic acid

Abstract

U ovom radu je provedena biokatalitička reakcija 1,4-karboligacije akrilonitrila i α-ketoglutarata uz dekarboksilazu MenD. Ispitan je utjecaj kofaktora magnezija i tiamin difosfata na početnu reakcijsku brzinu. Kinetika enzima je mjerena metodom početnih brzina, te je opisana dvosupstratnom Michaelis Menteničinom kinetikom. Postavljen je matematički model procesa u kotlastom reaktoru. Provedbom reakcije u kotlastom reaktoru uz 100 %-tni suvišak α-ketoglutarata, postignuta je potpuna konverzija akrilonitrila.Biocatalytical reaction of 1,4-addition of α-ketoglutarate and acrylonitrile catalyzed by decarboxylase MenD was carried out. The influence of cofactors magnesium and tiamin diphosphate on the initial reaction rate was tested. Kinetics of the enzyme was measured by the initial reaction rate method and was described by double substrate Michaelis-Menten kinetics. Mathematical model of the process in batch reactor was set. While preforming the reaction in a batch reactor with the α-ketoglutarate excess of 100%, complete conversion of acrylonitrile was achieved