Determination of vitamin C in different apple cultivars

Abstract

Ispitivane su četiri sorte jabuka, Fuji, Granny Smith, Gala i Cripps Pink, kupljene u trgovačkoj mreži. Provedena je analiza topljive suhe tvari, pH – vrijednosti i vitamina C na uzorcima kore, usplođa i srži. Topljiva suha tvar mjerila se digitalnim refraktometrom, a pH – vrijednost pH – metrom. Vitamin C određivao se tekućinskom kromatografijom visoke djelotvornosti (HPLC) s UV/Vis PDA detektorom. Rezultati analize pokazali su da usplođe sadrži najviše topljive suhe tvari (11.0 – 15.8 %) i najniži pH (3.31 – 3.97). Količina vitamina C varirala je ovisno o uzorku u rasponu od 0.03 – 6.35 mg/100 g uzorka. Najviše je bio prisutan u kori (ovisno o sorti od 0.61 – 6.35 mg/100 g), a najmanje u usplođu jabuke (od 0.03 – 0.06 mg/100 g). Količina vitamina C u srži je varirala od 0.04 – 0.11 mg/100 g. Prema dobivenim rezultatima za ukupnu količini vitamina C u cijelom plodu, sorte Granny Smith te Fuji sadržavale su najmanje (0,74 odnosno 2,01 mg/100g), a Cripps Pink te Gala najviše (5,85 odnosno 6,45 mg/100g) vitamina C.Four apple cultivars, Fuji, Granny Smith, Gala and Cripps Pink, purchased in chain store were analyzed to determine pH value, the quantity of soluble solids and vitamin C in samples of apple peel, pulp and core. Soluble solids were measured with digital refractometer and pH with pH – meter. Vitamin C was determined by high pressure liquid cromatography (HPLC) with UV/Vis PDA detector. The results showed that pulp contains the largest quantity of soluble solids (11.0 – 15.8 %) and the lowest pH (3.31 – 3.97). Content of vitamin C varied depending on the sample, and was in the range from 0.03 to 6.35 mg/100 g of sample. The highest amount was determined in the peel (depending on cultivar of 0.61 – 6.35 mg/100 g), and the lowest in the pulp (0.03 to 0.06 mg/100 g). The amount of vitamin C in the core varied from 0.04 to 0.11 mg/100 g Considering the amount of vitamin C in the whole apple fruit, cultivars Granny Smith and Fuji contained the least amount (0.74 and 2.01 mg/100 g, respectively), and Cripps Pink and Gala the highest amount (5.85 and 6.45 mg/100 g, respectively) of vitamin C