Rangelova, Desislava Yordanova

Generelt formål og hensigt Klassisk svinepest (CSF) er en meget smitsom sygdom, der har enorm betydning for økonomi og dyrevelfærd i svineproduktionen i hele verden. Generelt er vaccination en effektiv og sikker metode til at bekæmpe sygdommen. Efter vaccination udvikler grisens immunsystem antistoffer, disse er en væsentlig del af beskyttelsen. Imidlertid er den eneste kommercielle vaccine en levende afsvækket vaccine, der indeholder hel klassisk svinepestvirus (CSFV). Vaccination med denne vaccine fører til et antistof respons, som ikke kan skelnes fra antistofreaktion efter infektion. Anvendelse af disse vacciner, i tilfælde af udbrud, giver ikke mulighed for at påvise infektion blandt vaccinerede dyr (DIVA). For lande som Danmark, der har stor eksport af svin og svineprodukter, vil brugen af disse traditionelle vacciner, hindre muligheden for at påvise sygdomsfri status ved serologisk overvågning, idet alle vaccinerede grise vil være antistof positive. Dette ph.d.-projekt er en del af CSFV_goDIVA (bevilling nr. 227003), der er finansieret af EU FP7 med det hovedmål at udvikle og afprøve en ny DIVA vaccine kandidat og føre den frem til registrering. Vaccine kandidaten "CP7E2alf" er beregnet til intramuskulær vaccination af produktions svin eller til oral ”bait” vaccination af vildsvin. Som en del af den kliniske afprøvning af injektions vaccinen er effekten af "CP7E2alf" blev evalueret hos smågrise, der var positive for maternelle antistoffer, studiet danner basis for denne afhandling. Pattegrisene fik antistoffer med råmælk fra deres mødre vaccineret med traditionelle levende svækket vaccine C-stamme (Riems). De lovende resultater vedrørende sikkerhed og effekt af kandidaten DIVA vaccinen anviser nye muligheder for kontrol af et eventuel CSF udbrud. Brug af DIVA vaccine vil have kortere indflydelse på eksporten end brug af levende vaccine.General purpose and objectives Classical swine fever (CSF) is a highly contagious disease that causes huge economical losses and animal welfare concerns worldwide. Generally, vaccination is an effective and safe method to control the disease. Following vaccination the pig’s immune system develops antibodies that are significant part of the protection. However, vaccination with the only live attenuated vaccines existing on the market that contain a whole CSF virus (CSFV) with reduced infectivity, leads to production of an antibody response that does not differ from the antibody response developed after infection. Thus, implementation of these vaccines in case of outbreak will not give the possibility to differentiate infection in vaccinated animals (DIVA). For countries like Denmark, which are heavily dependent upon export of pigs and pig products the use of these traditional vaccines, will hamper the ability to proof a disease free status by serosurveillance, as all vaccinated piglets will be seropositive. This PhD-project is a part of an EU project (CSFV_goDIVA grant no 227003) that has been funded by the European Commission with a main goal to develop and test to a level of registration a new DIVA vaccine candidate. The vaccine candidate “CP7E2alf” is intended for either intramuscular vaccination of domestic pig or for bait vaccination of wild boar. In this thesis as part of the clinical testing of the injection vaccine the efficacy of “CP7E2alf” was evaluated in young piglets that were positive for maternally derived antibodies (MDA). These antibodies were obtained with colostrum from their mothers vaccinated with traditional live attenuated vaccine C-strain (Riems). The promising results concerning the safety and the efficacy of the candidate DIVA vaccine showed new opportunities for control of a possible CSF outbreak that will have reduced impact on the export.</p

Online Research Database In Technology

Third generation DIVA vaccine towards classical swine fever virus. Efficacy in face of maternal immunity

http://orbit.dtu.dk/ws/files/54900158/Desislava_Y_Rangelova_B5_contents_MARTS_2013_e_proof.pdf

Third generation DIVA vaccine towards classical swine fever virus. Efficacy in face of maternal immunity

Abstract

Similar works

Full text

Available Versions

Online Research Database In Technology