Lima Afonso Neto, Watson

Pirrung, Silvia

Schwarze, Daniel

Tufvesson, Pär

Vogel, Andreas

Woodley, John

Developing a biocatalytic transferase process involves many challenges, and choices have to be made regarding several aspects. Primarily, the selection of the biocatalyst itself and which biocatalyst formulation to use, but also the choice of amine donor has a decisive impact not only on reaction equilibrium, the inhibition profiles for substrates and products but also on the possibilities for in-situ product removal (ISPR) and technologies for shifting the equilibrium. In a challenging process such as the synthesis of optically pure chiral amines using ω-transaminase, these decisions will have a major influence on the process. Understanding these parameters and their effect on the process for the different reaction systems is important as it will help engineers make the right choices during process design. In this study we have therefore carried out an in depth characterization of different process parameters involved in the production of two chiral amines (S-methylbenzylamine and 3-amino-1-phenylbutane) (Figure 1) to demonstrate the effects of such decisions

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Dec 20, 2017Effect of critical process parameters on the synthesis of chiral aminesPirrung, Silvia; Lima Afonso Neto, Watson; Schwarze, Daniel; Vogel, Andreas; Woodley, John;Tufvesson, PärPublication date:2013Link back to DTU OrbitCitation (APA):Pirrung, S., Lima Afonso Neto, W., Schwarze, D., Vogel, A., Woodley, J., & Tufvesson, P. (2013). Effect ofcritical process parameters on the synthesis of chiral amines. Poster session presented at 1st InternationalSymposium on Transaminase Biocatalysis, Stockholm, Sweden.Effect of critical process parameters on the synthesis of chiral amines Silvia Pirrung1, Watson Neto1, Daniel Schwarze2, Andreas Vogel2, John M. Woodley1, Pär Tufvesson1 1Department of Chemical and Biochemical Engineering, Technical University of Denmark, Søltofts Plads, DK-2800 Lyngby, Denmark 2C-LEcta Gmbh, Leipzig, Germany e-mail: pt@kt.dtu.dk  Developing a biocatalytic transferase process involves many challenges, and choices have to be made regarding several aspects. Primarily, the selection of the biocatalyst itself and which biocatalyst formulation to use, but also the choice of amine donor has a decisive impact not only on reaction equilibrium, the inhibition profiles for substrates and products but also on the possibilities for in-situ product removal (ISPR) and technologies for shifting the equilibrium. In a challenging process such as the synthesis of optically pure chiral amines using ω-transaminase, these decisions will have a major influence on the process. Understanding these parameters and their effect on the process for the different reaction systems is important as it will help engineers make the right choices during process design. In this study we have therefore carried out an in depth characterization of different process parameters involved in the production of two chiral amines (S-methylbenzylamine and 3-amino-1-phenylbutane) (Figure 1) to demonstrate the effects of such decisions.                                  Figure 1: Overview of the reaction systems for production of S-methylbenzylamine and 3-amino-1phenylbutane [1] References: [1] Tufvesson, P.; Jensen, J. S.; Kroutil, W.; Woodley, J. M., Experimental determination of thermodynamic equilibrium in biocatalytic transamination. Biotechnol. Bioeng, 2012, 109, 2159-62 Keq=1/30 Keq=1/1.35 Keq=1/25 000 Keq=1/1647 

Effect of critical process parameters on the synthesis of chiral amines

http://orbit.dtu.dk/ws/files/53463572/Pirrung.pdf

Effect of critical process parameters on the synthesis of chiral amines

Abstract

Similar works

Full text

Available Versions

Online Research Database In Technology