Angelidaki, Irini

Borch, Martin Malthe

De Francisci, Davide

Holdt, Susan Løvstad

Møller, Per

Podevin, Mike

Online Research Database In Technology

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Downloaded from orbit.dtu.dk on: Dec 20, 2017Screening and Optimization of Case Specific Sustainable Mixotrophic MicroalgalMediumPodevin, Michael Paul Ambrose; Borch, Martin Malthe; De Francisci, Davide; Holdt, Susan Løvstad;Angelidaki, Irini; Møller, PerPublication date:2012Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Podevin, M., Borch, M. M., De Francisci, D., Holdt, S. L., Angelidaki, I., & Møller, P. (2012). Screening andOptimization of Case Specific Sustainable Mixotrophic Microalgal Medium. Poster session presented at  YoungAlgaeneer Symposium, Wageningen, Netherlands.DTU Environment, Department of Environmental Engineering,  Technical University of Denmark, Miljøvej, Building 113, 2800 Kgs. Lyngby, DENMARK The use of microalgae in wastewater treatment has been shown to be a compelling avenue towards sustainability. Dense algal cultures grown mixotrophically on carbon and nitrogen rich wastewater effluents have provoked increased interest following the wavering economic viability of photoautotrophic algae biomass productions. Recent work in our research group at the Technical University of Denmark addressed a new microtiter screening method evaluated on algal species capable of heterotrophic assimilation of carbon and nitrogen abundant in industrial wastes and their subsequent growth rate analysis. The aim was to test the method, find the right combination of industrial waste waters, bioremediate the waste waters and build up high added value product. Residuals of the biomass could potentially be utilized for bioenergy purposes. Abstract Michael Podevin1, Martin Malthe Borch2, Davide de Francisci, Susan Holdt, Irini Angelidaki, Per Moller   (1. Corresponding poster author: mipp@env.dtu.dk  2. Data acquisition: mmborch@gmail.com )   Developing a microtiter screening method  to identify viable  mixotrophic algae for the treatment of industrial waste.  Develop a microtiter screening protocol for testing algal growth viability on various waste media mixtures.  Analyze batch and chemostat cultivations with reference and mixed medium to validate results from the microtiter plates, and estimate the continuous growth potential.  Explore the potential of various mixtrophic algae in large scale reactors receiving various industrial waste water effluents. E4Water Project Aims Photos: Martin Borch Microtiter Strain Selection Selection of microalgal strains 1-2 microalgal species, and 1-2 microalgal consortiums will be identified for practicality based on wastewater criteria. Screening and Optimization of Case Specific Sustainable Mixotrophic Microalgae Medium Funded by: Genus Species Type Media Temp  °C pH Results Galdiera  sulphuraria red-algae FW-F/2 42 2 No Growth in tested conditions. Deeper green color than chlorella sp. Arthrospira sp. cyanobacteria Z8 26 6 to 11 Minimal growth, difficult to cultivate Chlorella  vulgaris green-algae MWC+Se, PP, Bold 15 7.5 No growth in tested conditions.  Phaeodactylum  tricornutum diatom L1 15 7 Fast dense growth in all growth conditions. Brown Color. Easy Cultivation and morphology and contamination visibility.  Pyrocystis  noctiluca diatom TL30 20 7 No growth in tested conditions and in 12:12h light:dark cycle in L1 and F/2 media. Chlorella  prototheocoides green-algae EG, EG:JM, Bristol-proteose, Proto-ENV 28 6 to 7 Fast dense growth for single condition it was tested in. Optimal pH for various waste substrates Can Achieve up to 64% lipids. Condition  Metabolism C-source (g/L) WO (%) Gly. (%) Pot. (%) Growth Rate (1/h) Color Reference-Glucose Mixotrophic 20 0 0 0 0.1322 Dark Green Reference-Glycerol Heterotrophic 20 0 0 0 0.1084 Light Green Balanced Low-Conc. Mixotrophic 0 0.5 0.35 2 0.0861 Dark Green N-Limited Low Mixotrophic 0 1 0.9 2.5 0.1007 Yellow N-Limited-High Heterotrophic 0 3 2.7 7.5 0.0579 Med. Green Scale-up Batch and Chemostat Fermentations Chemostat Method & Results Continuous operation was initiated after each batch cultivation exponential phase but could only be achieved for Chlorella prototheocoides grown mixotrophically  on glucose reference media   (see Figure-1). Table 1:  Several algal species were tested for their viability growing heterotrophically on various prepared media.  Table 2:  Chlorella prototheocoides grown under mixotrophic and heterotrophic metabolisms on a combination of waste media. Waste mixtures  may contain wet oxidized cow manure  (WO), crude glycerol (Gly.), or  potato water from potato factory (Pot.) Figure 1: Plot of chemostat off gas readings of Chlorella prototheocoides grown under mixotrophic conditions on glucose reference. Batch Method & Results (see Table-2). Mictrotiter Strain Selection Method & Results (See Table-1) Conclusions  A microtiter screening method identified Chlorella prototheocoides as a viable algal strain for experimentation.  A microtiter method can be used to estimate the growth of specific mixotrophic algal strains grown on industrial wastewater mixtures.  Chlorella prototheocoides can be grown under mixotrophic conditions in batch mode  on a combination of waste media , while achieving growth rates that may be competitive with glucose reference media.  Steady state cultivations of Chlorella prototheocoides are possible under reference glucose media but may risk contamination during purely heterotrophic metabolisms and both mixotrophic and heterotrophic metabolisms grown on waste mixtures.  Fouling of media containing wastewater mixtures must be addressed for continued development of this project. CO2 off gas (%) Age (h) 64h: chemostat initiated 125h: feed bottle change 125h: steady state Selection of wastewater streams for treatment with algae  Waste streams from external and internal partners in Kalundborg will be analyzed for their treatability by algae.  Scale-up  experiments   Selected microalgae  will be grown in single stage  continuous bioreactor configuration at a test facility. Solid-liquid separation  Simultaneous liquid phase separation and algal biomass quality will be evaluated.  Ecoduna PBR Pretreatment Industrial Wastewater  Pre-Breeding Harvester Biomass: Biofuels Biogas Fertilizer Animal feed Lipids  Carbohydrates Purified Water Discharge Recycle 

Screening and Optimization of Case Specific Sustainable Mixotrophic Microalgal Medium

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Screening and Optimization of Case Specific Sustainable Mixotrophic Microalgal Medium

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