Dalgaard, T.

Fink, Dorte Rosenbek

Jungersen, Gregers

Juul-Madsen, H. R.

Norup, L. R.

Schou, T. W.

Parasite infections are causing increasing concern in the poultry production industry, because the prevalence of several roundworms is rising. This is mainly due to changes in rearing systems, where the European Union ban of conventional cages for egg laying hens has led to an increase in the number of chicken flocks held in floor pens and free-range systems, which are associated with higher parasite burdens. In order to prevent infections with the nematode Ascaridia galli, development of a vaccine is desirable. In this study, three groups of 10 chickens were immunized with three different adjuvants together with a recombinant A. galli antigen. The adjuvants were CAF01, Emulsigen, and STV, and the antigen was Ag-NPA-1, a lipid-binding protein from the nematode polyprotein allergen/antigen family. Three immunizations were given i.m. with three-week intervals. A fourth group of 10 chickens was immunized with CAF01 and Ag-NPA-1, but only the first immunization was i.m., the next two immunizations were oral. A fifth group of 10 birds was injected i.m. with PBS as a control. The three groups that only received i.m. immunizations developed significantly higher Ag-NPA-1-specific serum IgG levels than the i.m./oral group and the control group. Three weeks after the last immunization, all animals were infected with 500 embryonated A. galli eggs, and 8 or 9 days post infection chickens were slaughtered and larvae numbers determined. No statistically significant differences in larvae numbers were observed between any of the groups, suggesting that the immunizations did not confer protection against A. galli infection

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                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Dec 20, 2017Immunization of chickens with a recombinant Ascaridia galli protein results inparasite-specific IgG with no protective effect against infectionVadekær, Dorte Fink; Schou, T. W. ; Norup, L. R.; Dalgaard, T.; Juul-Madsen, H. R. ; Jungersen, GregersPublication date:2012Link back to DTU OrbitCitation (APA):Fink, D. R., Schou, T. W., Norup, L. R., Dalgaard, T., Juul-Madsen, H. R., & Jungersen, G. (2012). Immunizationof chickens with a recombinant Ascaridia galli protein results in parasite-specific IgG with no protective effectagainst infection. Abstract from 4th European Veterinary Immunology Workshop (EVIW), Edinburgh, UnitedKingdom.Immunization of chickens with a recombinant Ascaridia galli protein results in parasite-specific IgG with no protective effect against infection  D. R. Fink 1, T. W. Schou2, L. R. Norup3, T. Dalgaard3, H. R. Juul-Madsen3, and G. Jungersen1 1National Veterinary Institute, Technical University of Denmark, Copenhagen, Denmark 2DHI, Hørsholm, Denmark 3Department of Animal Health and Bioscience – Immunology and Microbiology, Faculty of Agricultural Science, Aarhus University, Denmark   Parasite infections are causing increasing concern in the poultry production industry, because the prevalence of several roundworms is rising. This is mainly due to changes in rearing systems, where the European Union ban of conventional cages for egg laying hens has led to an increase in the number of chicken flocks held in floor pens and free-range systems, which are associated with higher parasite burdens. In order to prevent infections with the nematode Ascaridia galli, development of a vaccine is desirable. In this study, three groups of 10 chickens were immunized with three different adjuvants together with a recombinant A. galli antigen. The adjuvants were CAF01, Emulsigen, and STV, and the antigen was Ag-NPA-1, a lipid-binding protein from the nematode polyprotein allergen/antigen family. Three immunizations were given i.m. with three-week intervals. A fourth group of 10 chickens was immunized with CAF01 and Ag-NPA-1, but only the first immunization was i.m., the next two immunizations were oral. A fifth group of 10 birds was injected i.m. with PBS as a control. The three groups that only received i.m. immunizations developed significantly higher Ag-NPA-1-specific serum IgG levels than the i.m./oral group and the control group. Three weeks after the last immunization, all animals were infected with 500 embryonated A. galli eggs, and 8 or 9 days post infection chickens were slaughtered and larvae numbers determined. No statistically significant differences in larvae numbers were observed between any of the groups, suggesting that the immunizations did not confer protection against A. galli infection.  

Immunization of chickens with a recombinant Ascaridia galli protein results in parasite-specific IgG with no protective effect against infection

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Immunization of chickens with a recombinant Ascaridia galli protein results in parasite-specific IgG with no protective effect against infection

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