Frandsen, Henrik Lauritz

Licht, Tine Rask

Skov, Thomas Hjort

Smedsgaard, Jørn

Sulek, Karolina

Wilcks, Andrea

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Dec 20, 2017The effect of different in vitro conditions on the metabolic footprint of Lactobacillusacidophilus NCFMSulek, Karolina; Wilcks, Andrea; Licht, Tine Rask; Smedsgaard, Jørn; Skov, Thomas Hjort; Frandsen,Henrik LauritzPublication date:2010Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Sulek, K., Wilcks, A., Licht, T. R., Smedsgaard, J., Skov, T. H., & Frandsen, H. L. (2010). The effect of differentin vitro conditions on the metabolic footprint of Lactobacillus acidophilus NCFM. Abstract from InternationalScientific Conference Probiotics and Prebiotics, Kosice, Slovakia.The effect of different in vitro conditions on the metabolic footprint of Lac-tobacillus acidophilus NCFM  Sulek, Karolina; Wilcks, Andrea; Licht, Tine Rask; Smedsgaard, Jørn; Skov, Thomas Hjort; Frandsen, Henrik Lauritz  National Food Institute, Technical University of Denmark (DTU Food), Mørkhøj By-gade 19, 2860 Søborg, Denmark, kasul@food.dtu.dk   Introduction  During the last decade, it has become evident that the complex ecosystem of mi-crobes inhabiting the human gut plays an important role for human health. An in-creasing number of publications have shown that the composition and activity of our intestinal microbiota affects a number of different so-called lifestyle diseases including allergy, obesity, and colorectal cancer, as well as our susceptibility to intestinal infec-tions and inflammation.  Additionally, it has become evident that the intestinal microbiota can be modulated by intake of pre- and probiotics. A large number of studies have addressed the effects of dietary interventions on the presence of specific bacterial metabolites, which are an-ticipated to play a role for gut health. However, such data evidently provide only small parts of the complex puzzle constituting the interactions between diet, microbiota, and mammalian host.  This project’s objective is to elucidate the mechanism behind the beneficial effects of pre- and probiotics. This will lead to development of new pre- and probiotics targeting specific lifestyle related disorders.   Methods  In vitro fermentation tests in an anaerobic chamber were performed with Lactobacillus acidophilus NCFM grown under different conditions (e.g. pH, addition of prebiotics) in a semi-synthetic medium. Direct infusion mass spectrometry (MS) and liquid chroma-tography (LC) combined with MS is used for metabolite footprinting, performed in mi-croTOF-Q Mass Spectrometer. Newly developed advanced (‘omics-‘) methodologies allow analysis of biological interactions, which include statistical approach to bacterial metabolome such as principal component analysis.        Results  In vitro fermentation studies showed that pH value has a significant effect on the Lac-tobacillus acidophilus NCFM growth and metabolite footprint. Quantitative and quali-tative differences in extracellular metabolite were observed by use of both direct infu-sion MS and LC-MS. Principal component analysis will be used for visualization of the exact metabolite variations in the samples. It is expected that later analyses will re-veal specific metabolite production by Lactobacillus acidophilus NCFM induced by the presence of varied prebiotic carbohydrates in the semi-synthetic medium.     Discussion  The production of metabolites in vitro by Lactobacillus acidophilus NCFM can be af-fected by pH value. The same method will later on be used for studying the effect of prebiotics on the metabolite footprint. This would also allow analysis of the prebiotic candidates’ beneficial potential. Future studies will include experiments in gnotobiotic mice, mono-colonized with Lactobacillus acidophilus NCFM. Production of specific metabolites from the bacterium and the host epithelium will be investigated as well as the metabolite profile in blood. 

The effect of different in vitro conditions on the metabolic footprint of Lactobacillus acidophilus NCFM

http://orbit.dtu.dk/ws/files/9442476/prod21338281751865.Abstract_IPC2010_new_type.pdf

The effect of different in vitro conditions on the metabolic footprint of Lactobacillus acidophilus NCFM

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