Aalberse R. C.

Albrecht M.

Antoni G.

Arnon R.

Astwood J. D.

Atassi M. Z.

Bannon G. A.

Barkholt V.

Barlow D. J.

Brons N. H. C.

Burks A. W.

Bøgh K. L.

Charlotte B. Madsen

Ditto A. M.

Dupont D.

E. N. Clare Mills

EFSA Panel on Genetically Modified Organisms (GMO).

Eiwegger T.

Fehlner P. F.

Fu T. J.

Grundy J.

Halassy B.

Hourihane J. O.

Huby R. D. J.

Katrine L. Bøgh

Kenna J. G.

King T. P.

Kroghsbo S.

Lack G.

Lin J.

Maleki S. J.

Mandalari G.

Mills E. N.

Morein B.

Moreno F. J.

Morisawa Y.

Muller S.

Neil M. Rigby

Partidos C. D.

Poulsen O. M.

Roth-Walter F.

Sarobe P.

Schimek E. M.

Scholl I.

Shaw D. M.

Shin D. S.

Sicherer S. H.

Taylor S. L.

Thomas K.

Untersmayr E.

Van Beresteijn E. C. H.

van Boxtel E. L.

Van Regenmortel M. H.

Van Regenmortel M. H. V.

Vibeke Barkholt

Wichers H. J.

The major peanut allergen Ara h 1 is an easily digestible protein under physiological conditions. The present study revealed that pepsin digestion products of Ara h 1 retained the sensitizing potential in a Brown Norway rat model, while this sensitizing capacity was lost by separating the digest into fractions by gel permeation chromatography. Protein chemical analysis showed that the peptide composition as well as the aggregation profiles of the fractions of Ara h 1 digest differed from that of the whole pool. These results indicate that the sensitizing capacity of digested Ara h 1 is a consequence of the peptides being in an aggregated state resembling the intact molecule or that most peptides of the digests need to be present in the same solution, having a synergistic or adjuvant effect and thereby augmenting the immune response against other peptides

Bøgh, Katrine Lindholm

Barkholt, Vibeke

Rigby, Neil M.

Mills, E. N. Clare

Madsen, Charlotte Bernhard

Online Research Database In Technology

Digested Ara h 1 Loses Sensitizing Capacity When Separated into Fractions

Crossref

Journal of Agricultural and Food Chemistry

http://orbit.dtu.dk/ws/files/7729241/PDF69.pdf

Digested Ara h 1 Loses Sensitizing Capacity When Separated into Fractions

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Online Research Database In Technology

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