Synthesis, application in biotransformations and cytotoxicity of selected imidazolium-based ionic liquids

Abstract

Pripravljene nove imidazolijeve ionske tekućine ispitane su kao otapala u lipazom kataliziranoj sintezi izoamil-acetata te je uspostavljena visokoproduktivna kontinuirana sinteza izoamil-acetata u mikroreaktoru s nasutim slojem imobilizirane lipaze (61 mmol L−1 min−1). Primjenom nekonvencionalnih metoda u pripravi ionskih tekućina moguće je znatno skratiti vrijeme reakcije i smanjiti potrošnju energije. Ispitivanjem citotoksičnosti na CCO staničnoj liniji utvrđeno je da imidazolijeve ionske tekućine uglavnom ispoljavaju umjerenu toksičnost (EC50 = 0,1 – 10 mmol L-1), induciranu oštećenjem stanične membrane i posljedičnom nekrozom.Novel imidazolium ionic liquids were prepared and tested as solvents for enzyme-catalyzed isoamyl acetate synthesis and highly productive continuous esterification in an immobilized lipase packed-bed microreactor was developed (61 mmol L−1 min−1). The use of unconventional methods for ionic liquids’ synthesis contributes to the reduction of preparation time as well as energy consumption. Results of in vitro cytotoxicity assays indicate moderate toxicity of imidazolium ionic liquids (EC50 = 0,1 – 10 mmol L-1) through the mechanism of membrane disruption followed by necrosis