Synthesis and evaluation of fluorescent probe for H2S sensing

Abstract

Sumporovodik ($H_{2}S$) je prvenstveno poznat kao toksin i njegova biološka uloga često nije prepoznata, međutim $H_{2}S$ se javlja u organizmima, od bakterija do sisavaca, kao redoks aktivna signalna molekula, odnosno biološki glasnik. Njegova koncentracija u organizmima iznosi 10-100 μmol/$dm^{3}$ i povezana je s različitim patološkim procesima. Zbog tako male koncentracije potrebne su novije i osjetljivije metode detekcije. Problem s određivanjem $H_{2}S$ u živim organizmima je brz katabolizam zbog kojeg dolazi do stalne promjene njegove koncentracije i posljedica toga su netočni rezultati. Kao jedno od rješenja tih problema, predlažu se metode bazirane na fluorescenciji. Cilj ovog rada bio je sintetizirati reverzibilno fluorescentno osjetilo za $H_{2}S$ te okarakterizirati dobiveni spoj. Pripravljena su tri konačna produkta: $Se-P_{1}$, $Se-P_{2}$ i $P_{1}-NaN_{3}$, a njihova karakterizacija provedena je FT-IR spektroskopijom i fluorescentnom spektroskopijom. Navedenim metodama utvrđeno je kako je sintezom $Se-P_{1}$ dobiveno reverzibilno fluorescentno osjetilo za $H_{2}S$, sinteza $Se-P_{2}$ se pokazala neuspješnom, a sintezom $P_{1}-NaN_{3}$ dobiveno je reverzibilno pH osjetilo, ali ne i osjetilo za $H_{2}S$.Hydrogen sulfide ($H_{2}S$) is commonly known as a toxin and its biological role is often neglected. $H_{2}S$ is a redox active signal molecule and can be found in different organisms, from bacteria to mammals, where it has a role of a biological messenger. Its concentration in live organisms is 10-100 μmol/$dm^{3}$ and can be connected with different pathological processes. Hence, the new and more sensitive detection methods are necessary. The main problem when it comes to $H_{2}S$ detection is its fast catabolism, which means that the concentration is constantly changing, giving us the incorrect results. As one of the possible solutions, fluorescence based methods are suggested. The aim of this paper was to synthesize a reversible fluorescent probe for $H_{2}S$ sensing and characterize the compound. Three final products were made:$Se-P_{1}$ , $Se-P_{2}$ i $P_{1}-NaN_{3}$, and were characterized using FT-IR spectroscopy and fluorescence spectroscopy. These methods showed that a reversible fluorescent probe for $H_{2}S$ sensing was indeed synthesized ($Se-P_{1}$). $Se-P_{2}$ synthesis wasn't successful and it was shown that the $P_{1}-NaN_{3}$ works as a reversible pH probe, but not an $H_{2}S$ probe