Influence of pH of nutrition media on growth of Sclerotium cepivorum

Abstract

Gljiva Sclerotium cepivorum je zemiljišni i fakultativni parazit, uzrokuje velike štete na Allium vrstama. Uzročnik je bijele truleži luka i češnjaka. Cilj istraživanja bio je utvrditi utjecaj temperature i pH vrijednosti hranjive podloge krumpira-krumpir dekstrozni agar (PDA) na rast micelija gljive Sclerotium cepivorum. Pokus je proveden u in vitro uvjetima u četiri ponavljanja. Podloge su bile nacjepljene u 36 petrijevih zjedlica pri tri pH vrijednosti (5,5; 6,5; 8,0) te u termostat komori na temperaturama 15, 22 i 30°C i svjetlosnom režimu 24 sata tama. Porast micelija mjeren je tri puta tijekom deset dana. Najbolji razvoj micelija gljive bio je pri 15 i 22 °C i navedenim pH vrijednostima podloge, dok se micelij pri temperaturi od 30 °C i sve tri pH vrijednostima podloga nije razvio.The fungus Sclerotium cepivorum is soil and facultative parasite that causes great damage to Allium species. The causative agent of white rot of onion and garlic. The aim oft his study was to determine the infuence of temperature and pH on the growth oft he mycelium fungus Sclerotium cepivorum. The experiment was performed in vitro with four repetitions. The fungus was grown at temperatures of 15, 22 and 30 °C, using 24 hours of light and dark at a pH 5.5, 6.5, 8.0 PDA substrate. Mycelium growth was measured three times over ten days. Mycelium successfully developed at 15 and 22 °C and all pH values and the mycelium at a temperature of 30 °C and the pH values of all substrate is not developed