University of Split. School of Medicine. Medical chemistry and biochemistry.
Abstract
Cilj istraživanja: Cilj je istraživanja ispitati potencijalno citotoksično djelovanje polisulfida, spojeva izoliranih iz biljne vrste Allium spp., na humane karcinomske stanice mokraćnog mjehura (UM-UC-3) i glioblastoma (LN229). Pretpostavka je da će se broj karcinomskih stanica, nakon izlaganja polisulfidima, broj smanjiti u odnosu na kontrolnu skupinu.
Materijali i metode: Ispitivanje citotoksičnosti na staničnim linijama karcinoma mokraćnog mjehura i glioblastoma rađeno je MTT testom. Usporedbom apsorbancije nastalog formazana kod stanica tretiranih polisulfidimai apsorbancije kod netretiranih stanica (kontrola), dobili smo postotak preživljenja karcinomskih stanica. Citotoksičnost se određivala nakon 4, 24, 48 i 72 sata.
Rezultati:
Rezultati su prikazani grafički u odnosu vremena inkubacije i postotka metabolički aktivnih stanica.
Kod stanica karcinoma mokraćnog mjehura svi polisulfidi ostvaruju bolji učinak pri višim koncentracijama od 50 μg/mL i 100 μg/mL, pri različitim vremenima inkubacije. Najznačajniji učinak na smanjenje metabolički aktivnih stanica imaju dialil disulfid i dipropil trisulfid pri koncentraciji od 100 μg/mL nakon 72h inkubacije.
Kod stanica glioblastoma većina polisulfida najjači citotoksični učinak ima pri višim koncentracijama od 50 μg/mL i 100 μg/mL,pri različitim vremenima inkubacije. Najznačajniji učinak na smanjenje metabolički aktivnih stanica imaju dialil disulfid i dipropil trisulfid pri koncentraciji od 100 μg/mL nakon 72h inkubacije.
Zaključci:
In vitro izlaganje stanica karcinoma mokraćnog mjehura (UM-UC-3) i glioblastoma (LN229) polisulfidima dovodi do smanjenja preživljenja tih stanica. Citotoksični učinak ovisan je o vremenu inkubacije i koncentraciji. Djelovanje polisulfida nije uvijek razmjerno povećanju koncentracije i vremenu inkubacije, te u pojedinim slučajevima dolazi do oporavka stanica. Citotoksični učinak polisulfida, što je ujedno i hipoteza našeg ispitivanja, je potvrđen, a idući korak je potvrđivanje tih učinaka in vivo ispitivanjem na modelima karcinoma mokraćnog mjehura i glioblastoma kod životinja.The aim of the research:
The aim of the research is to examine the potential cytotoxic effects of polysulfides, a chemical substance isolated from the Allium spp. family of plants on human carcinoma cells of urinary bladder (UM-UC-3) and glioblastoma (LN229). The assumption is that after the exposure of carcinoma cells to polysulfides their number will be reduced in comparison to the control group.
Materials and methods:
MTT assay was used to establish cytotoxicity levels on urinary bladder and glioblastoma cancer cell lines. The comparison between the absorbance of created formazan in cells treated with polysulfides and those untreated (the control group) established the survival rates of carcinoma cells. Cytotoxicity levels were established after the periods of 4, 24, 48 and 72 hours.
Results:
The results are graphically presented in relation to incubation times and the percentages of metabolically active cells.
In urinary bladder carcinoma cells all polysulfides show better effects in higher concentrations of 50 μg/mL and 100 μg/mL, with different incubation times. The most prominent effect on the reduction of metabolically active cells is achieved by diallyl disulfide and dipropyl trisulfide at the concentration of 100 μg/mL after 72-hour incubation time.
In glioblastoma cells most polysulfides have the strongest effect in higher concentrations of 50 μg/mL and 100 μg/mL, with different incubation times. The most significant effect on the reduction of metabolically active cells is achieved by diallyl disulfide and dipropyl trisulfide at the concentration of 100 μg/mL after 72-hour incubation time.
Conclusions:
In vitro exposure of urinary bladder carcinoma cells (UM-UC-3) and glioblastoma (LN229) to polysulfides leads to reduced cell survival rate. Cytotoxic effects depend on incubation time and concentration. Polysulfides effect is not always proportional to the increase in concentration and incubation time and in some cases cell recovery occurs. The cytotoxic effect of polysulfides is confirmed, which was the hypothesis of the research, and the next step is the confirmation of these effects by in vivo studies on the models of animal urinary bladder and glioblastoma cancers
