University of Split. School of Medicine. Endodoncy and restorative dentistry.
Abstract
Cilj istraživanja: Dentalni tehničari redovito su izloženi metalnoj prašini i monomerima akrilnih smola koji mogu ozbiljno ugroziti njihovo zdravlje. Cilj ove presječne studije bio je procijeniti i usporediti pojavnost mikronukleusa i drugih jezgrinih anomalija na stanicama bukalne sluznice dentalnih tehničara.
Materijali i metode: Studija je provedena na 45 dentalnih tehničara, te 50 doktora dentalne medicine i 50 dentalnih asistenata kao kontrolnih skupina. Sve grupe su podudarne prema dobi. Oštećenje DNA analizirano je mikronukleus testom.
Rezultati: Rezultati su pokazali da doktori dentalne medicine i dentalni asistenti imaju znatno nižu učestalost broja stanica s mikronukleusom u usporedbi sa dentalnim tehničarima (0,68 ± 0,74, 0,58 ± 0,81 i 1,58 ± 2,07, P = 0,008 i 0,004), kariolize (0,10 ± 0,30, 0,20 ± 0,49 i 1,42 ± 1,25, P = 0,000 i P = 0,000), kondenziranog kromatina (0,16 ± 0,37, 0,14 ± 0,35 i 0,76 ± 0,98, P = 0,000 i P = 0,000) i piknoze (0,04 ± 0,20, 0,08 ± 0,27 i 0,96 ± 1,24, P = 0,000 i P = 0,000).
Zaključak: Rezultati ovog istraživanja pokazali su povećan broj citogenetskih oštećenja u stanicama bukalne sluznice dentalnih tehničara u odnosu na kontrolne skupine, što se može povezati s neadekvatnim zaštitnim mjerama na radu i izloženosti štetnim tvarima iz materijala kojima se koriste u svakodnevnom radu.Objectives: Dental technicians are regularly exposed to metal dust and monomers from acrylic resins that can seriously jeopardize their health. The aim of this cross sectional study was to assess the incidence of micronuclei and other nuclear abnormalities in buccal mucosal cells of dental technicians.
Materials and methods: Study was conducted on 45 dental technicians, 50 doctors of dental medicine and 50 dental assistants who were used as the control group. All groups were match according to age. DNA damage was analysed by micronucleus assay.
Results: The results showed that the incidence of number of cells with micronuclei is significantly lower in doctors of dental medicine compared to dental technicians (0.68 ± 0.74, 0.58 ± 0.81 and 1.58 ± 2.07; P = 0.008 and 0.004, respectively). The same pattern of incidence was demonstrated for karyolitic cells (0.10 ± 0.30, 0.20 ± 0.49 and 1.42 ± 1.25; P = 0.000 and P = 0.000, respectively), condensed chromatin (0.16 ± 0.37, 0.14 ± 0.35 and 0.76 ± 0.98; P = 0.000 and P = 0.000, respectively) and pyknotic cells (0.04 ± 0.20, 0.08 ± 0.27 and 0.96 ±1.24; P = 0.000 and P = 0.000, respectively).
Conclusion: The results of this study have shown an increased number of cytogenetic defects in dental technician buccal mucosa compared to control groups that can be associated with inadequate protective measures at work and exposure to harmful ingredients from the materials that are used in everyday work