Aarestrup, Frank Møller

Aidara-Kane, Awa

Hendriksen, Rene S.

Jensen, Arne Bent

Karlsmose, Susanne

Mikoleit, Matthew

Wong, Danilo M.A. Lo Fo

Online Research Database In Technology

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Downloaded from orbit.dtu.dk on: Dec 19, 2017WHO Global Foodborne Infections Network external quality assurance system (EQAS)for antimicrobial susceptibility testing of Salmonella isolatesKarlsmose Pedersen, Susanne; Hendriksen, Rene S.; Mikoleit, Matthew; Jensen, Arne Bent; Aidara-Kane, Awa; Wong, Danilo M.A. Lo Fo; Aarestrup, Frank MøllerPublication date:2011Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Karlsmose, S., Hendriksen, R. S., Mikoleit, M., Jensen, A. B., Aidara-Kane, A., Wong, D. M. A. L. F., &Aarestrup, F. M. (2011). WHO Global Foodborne Infections Network external quality assurance system (EQAS)for antimicrobial susceptibility testing of Salmonella isolates. Poster session presented at IMED 2011International Meeting on Emerging Diseases and Surveillance, Vienna, Austria.20%30%40%50%60%70%80%90%100%020406080100120140160Participants% of participants uploading at least one result for the QC-strain% of participants with all values within the QC-rangeEQAS iteration, YearNumberPercentWHO Global Foodborne Infections Network external quality assurance system (EQAS) for antimicrobial susceptibility testing of Salmonella isolatesConclusionsThe results from the GFN EQAS, one of the largest of its kind in the world, show that most laboratories worldwide are capable of correctly performing AST of the Salmonella isolates included in the test panel. However, this study also indicates a continuing need for improvement. Future training efforts should be aimed at enhancing the quality control of the assay stressing the importance of the QC strain, the true indicator of the quality of AST performance, and at enhancing the ability to detect emerging resistance phenotypes, e.g. plasmid mediated quinolone resistance and ESBL.Susanne Karlsmose1, Rene S. Hendriksen1, Matthew Mikoleit2, Arne B. Jensen1, Awa Aidara-Kane3, Danilo M.A. Lo Fo Wong3, Frank M. Aarestrup1, and WHO GFN Steering Committee Members1 WHO Collaborating Centre for Antimicrobial Resistance in Foodborne Pathogens and EU Reference Laboratory for Antimicrobial Resistance, National Food Institute, Copenhagen, Denmark2 WHO Collaborating Centre for Surveillance, Epidemiology and Control of Salmonella and other Foodborne Diseases, Centers for Disease Control and Prevention, Division of Foodborne, Waterborne, and Environmental Diseases, Atlanta, GA, USA3 World Health Organization, Department of Food Safety, Zoonoses and Foodborne Diseases, Geneva, SwitzerlandMethodsNine EQAS iterations were conducted between 2000 and 2009.  In each iteration, participants received eight test strains and  new participants were provided with the E. coli ATCC 25922 quality control (QC) strain. The test strains were selected to cover a variety of antimicrobial resistance profiles, especially emerging phenotypes like plasmid-mediated quinolone resistance and extended-spectrum beta lactamase-producers (ESBL).In each iteration, participating laboratories submitted AST results for eight Salmonella isolates through a secured website and received an instant report with suggestions for corrective actions if needed.BackgroundThe WHO Global Foodborne Infections Network (GFN) is led by 11 steering committee partners (Figure 1) and builds global capacity to conduct integrated, laboratory-based surveillance, detection and response to outbreaks of foodborne and other infectious enteric diseases through a number of components. One such component is the global External Quality Assurance System (EQAS) for antimicrobial susceptibility testing (AST) of Salmonella species which was initiated by GFN in 2000. EQAS enhances the capacity of national reference laboratories to obtain reliable data for monitoring and surveillance purposes worldwide.ResultsA total of 323 laboratories in 119 countries participated in the AST component of at least one EQAS iteration conducted between 2001-2009.(Figure 3)Cumulatively, 92% of the uploaded AST results on the test isolates were in agreement with expected results.The percentage of laboratories uploading data for the QC strain ranged by iteration from 72% to 99% with 41% to 56% of the laboratories having all values within accepted limits (Figure 3).Data from 2001 to 2007 are summarized and published1.Figure 3: Marked with color are the countries from which one or more laboratories have participated in the WHO GFN EQAS in any of the iterations from 2001 to 2009 (the 2000-data are not included as they were not uploaded to the database)Technical University of Denmark, National Food Institute, Susanne Karlsmose (suska@food.dtu.dk) International Meeting on Emerging Diseases and Surveillance (IMED 2011), February 4-7, 2011, Vienna, AustriaFigure 1: The GFN steering committee partnersReference1 Results of Use of WHO Global Salm-Surv External Quality Assurance System for Antimicrobial Susceptibility Testing of Salmonella Isolates from 2000 to 2007. Hendriksen RS, Seyfarth AMS, Jensen AB, Whichard J, Karlsmose S, Joyce K, Mikoleit M, DeLong SM, Weill FX, Aidara-Kane A, Lo Fo Wong DM, Angulo FJ, Wegener HC, Aarestrup FM. J Clin Microbiol, 2009 Jan; 47:79–85Figure 2: Participating laboratories testing the QC-reference strain (E. coli ATCC 25922)Poster  12.022  

WHO Global Foodborne Infections Network external quality assurance system (EQAS) for antimicrobial susceptibility testing of Salmonella isolates

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WHO Global Foodborne Infections Network external quality assurance system (EQAS) for antimicrobial susceptibility testing of Salmonella isolates

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