Borowicki, A.

Brenner-Weiß, G.

Glei, M.

Neidig, A.

Obst, U.

Scheu, K.

Stein, K.

Wachter, N.

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Effect of wheat aleurone on the microbial gut flora and on the formation of SCFA after simulated gastro-intestinal passage

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EFFECT OF WHEAT ALEURONE ON THE MICROBIAL GUT FLORA AND ON THE FORMATION OF SCFA AFTER SIMULATED GASTRO-INTESTINAL PASSAGE1 Institute of Functional Interfaces (IFG), Department of Microbiology of Natural and Technical Interfaces, Research Centre Karlsruhe, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany; kerstin.scheu@ifg.fzk.de2 Institute for Nutrition, Department of Nutritional Toxicology, Friedrich-Schiller-University Jena, Dornburger Str. 24,07743 Jena, Germany; Michael.Glei@uni-jena.de3 Kampffmeyer Food Innovation GmbH, Trettaustr. 32-34, 21107 Hamburg, Germany; Norbert.Wachter@Kampffmeyer.deScheu, Kerstin1; Neidig, Anke1; Brenner-Weiß, Gerald1; Stein, Katrin2; Borowicki, Anke2; Glei, Michael2; Wachter, Norbert3; Obst, Ursula1INTRODUCTIONDietary fibre, such as wheat aleurone, can support microbial fermentation in the gastro-intestinal tract resulting in a higher content of health-relevant metabolites, e.g. short chain fatty acids (SCFA), which contribute to colon cancer prevention. In the present study, digestion was simulated in vitro with conditions adjusted to gastro-intestinal passage using wheat aleurone. After simulation of the gastro-intestinal passage DNA extracts of the pelletized faecal samples were subjected to qPCR for the enumeration of total Eubacteria, Lactobacilli and Bifidobacteria. The supernatant was used for quantification of SCFA by GC-MS. METHODIC APPROACHEnumeration of total Eubacteria, Lactobacilli and Bifidobacteria was gained byqPCR using species specificprimer systems. Detection was done with SYBR Green.RESULTSIn vitrodigestionqPCRGC-MSCONCLUSIONSCFA were quantified by GC-MS after liquid-liquid extraction with tBME and derivatisation with 10%TMSHRelating to the total eubacterial cell counts in the presence of wheat aleurone the number of Bifidobacteria in the faeces pellet was increased from 0.37 % (control) to 6.28 % of the total bacterial counts.At the same time the supernatant of wheat aleurone contained 1.6 to 3.8 fold higher amounts of SCFA than the control. Moreover, the molar ratio of SCFA in the sample with wheat aleurone was shifted in favour of butyrate.As long as Bifidobacteria are able to metabolize complex carbohydrates, it is obvious that the cell counts of Bifidobacteria are higher in the sample with the digestion of wheat aleurone. By contrast, Lactobacilli are particulary prevalent in the upper gastrointestinal tract and rather ferment simple sugars. This explains the only slight increase in their cell counts. Total SCFA especially butyrate are enhanced in the sample with wheat aleurone. This is an important factor because butyrate prevents colon cancer by initiating cell differentiation and apoptosis. To enhance gut health, a fibre-rich diet (e.g. with aleurone-enriched products) could have a prebiotic effect by increasing the cell counts of Bifidobacteria and resulting in a higher amount of SCFA. 10g/L fermentable sample +fermentation buffermouth simulation (α-amylase, 5 min)stomach simulation (pepsin, pH 2, 2 h)duodenum simulation including dialysis(intestinal extract, pH 6.5, 6 h)colon simulation (pH 6.5, 24 h)faeces inoculationThis work was funded by a grant of the BMBF (0313829B).In comparison to the control, cell counts of Bifidobacteria in the faeces pellet were enhanced a hundredfold with the digestion of wheat aleurone. Lactobacilli were slightly influenced by the simulated digestion with wheat aleurone. 1,49E+071,30E+090,00E+002,00E+084,00E+086,00E+088,00E+081,00E+091,20E+091,40E+091,60E+09control wheat aleuronecells per 500 µL fermentation pelletBifidobacteriashort chain fatty acids0510152025control wheat aleuronemmol/ Lacetate propionate butyrate2,07E+063,02E+070,00E+005,00E+061,00E+071,50E+072,00E+072,50E+073,00E+073,50E+07control wheat aleuronecells per 500 µL fermentation pelletLactobacillicontrol wheat aleuronetotal Eubacteria [%] 99.57 ±19.65 93.56 ± 0.94Lactobacilli [%] 0.02 ± 0.00 0.05 ± 0.00Bifidobacteria [%] 0.37 ± 0.21 6.28 ± 0.44

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Effect of wheat aleurone on the microbial gut flora and on the formation of SCFA after simulated gastro-intestinal passage

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