Brändle, M.

Frey, W.

Gusbeth, C.

Obst, U.

Schwartz, T.

Wuestner, R.

English

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KIT – University of the State of Baden-Wuerttemberg andNational Research Center of the Helmholtz AssociationProblem AimDissemination of hygienically relevant multi-resistant pathogens from clinical wastewaters into the downstream wastewater systems and the aquatic environmentConventional disinfection techniques demonstrate disadvantages like toxic by-products (chemical disinfection) or reduced efficiency due to high particle load (UV disinfection) Wastewaters contribute to horizontal gene transfer of resistance plasmids or virulence factors due to the high number of multi-resistant pathogensAlternative wastewater disinfection techniqueefficient reduction of microbial contaminants at local points upstream of municipal wastewater treatment plantsno application of chemicals so no toxic technique derived by-products are generatedno affection of the natural occurring nuclease activity being important for the break down of extracellular DNA like resistance genes on transferable plasmidsPulsed electric field treatment (PEF)Monitoring of Inactivation Efficiencies and Regeneration Potential of Antibiotic Resistant E. faecium during Pulsed Electric Field Treatment of WastewaterM. Brändle1, C. Gusbeth2, R. Wuestner2, U. Obst1, W. Frey2, T. Schwartz11Karlsruhe Institute of Technology, Institute of Functional Interfaces, Department of Microbiology of Natural and Technical Surfaces, Germany 2Karlsruhe Institute of Technology, Institute for Pulsed Power and Microwave Technology, GermanyCONCLUSIONnovel technology for the treatment of microbial charged wastewater like clinical wastewaterPEF treatment is efficient to eliminate natural occurring wastewater bacteria, including antibiotica resistant strainssynergistic effects are strain-dependentbacteria are able to repair some damages resulting in a lesser inactivation rate, probably by inducing pbp5 and recAPEFMode of function: directed against membranes of biological cells; the bi-electrical breakthrough of the phospholipid double layer induced by pulsed electric fields causes cell disruptionPresent application areas: biotechnology (cell disruption, extraction of intracellular products), food industry (sterilization, wine production)New application area           Disinfection of microbial charged wastewaterstrain 1885012345670 40 80energy [J/mL]reduction [orders of magnitude]20°C 55°C simultaneous 55°C sequential0,32,10,80,21,50,4control4,14,43,63,25,23,880J/mL3,12,92,21,43,92,040J/mLsequentiell treatment (heat, then PEF treatment)5,15,94,54,14,74,680J/mL44,23,72,93,43,340J/mLsimultaneos treatment (PEF treatment and heat together)strain1885strain1435strain67/08strain39/05strain1319ATCC6057reduction [orders of magnitude]strain 1885012345670 40 80energy [J/mL]reduction [orders of magnitude]20°C 55°C 20°C after 24h 55°C after 24hRESULTSInactivation efficiencies of PEF treatment and synergistic effects• antibiotic resistant strains were inactivated with up to 6 decimal orders of magnitude reduction at sublethal temperatur (55°C)     • complete reduction was determined at 60°C• significant synergistic effects between PEF and sublethal temperature could be detected and were strain-dependentRegeneration and stress responses24 h after PEF treatment inactivation efficiency was declined about one order of magnitude (40 J/mL) and two orders of magnitude (80 J/mL) during simultaneous treatment at 55°Crepair mechanismsstress responseATCC 6057012345670 40 80energy [J/mL]reduction [orders of magnitude]20°C 55°C simultaneous 55°C sequentialthe pbp5 gene playing a major role in peptidoglycan synthesis was induced in all antibiotic-resistant bacteria, but not in the reference strainrecA -expression 0123456780 30 60 90 120 150time [min]induction factorthe recA gene involved in repairing DNA injuries was induced by timepbp5 -expression024681012141618201 2 1 2 1 2 1 2 1 2 1 2induction factor1 2 1 2 1 2 1 2 1 2 1 2treatment with 1: 40 J/mL, 2: 80 J/mLATCC 6057strain67/05strain39/05strain1319strain1435strain1885

Monitoring of inactivation efficiencies and regeneration potential of antibiotic resistant E. faecium during pulsed electric field treatment of wastewater

https://publikationen.bibliothek.kit.edu/220079096/3815684

Monitoring of inactivation efficiencies and regeneration potential of antibiotic resistant E. faecium during pulsed electric field treatment of wastewater

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