Nielsen, Michael Engelbrecht

Wulff, Tune

Annexins is a diverse family of structural related proteins with 12 known members in humans. Interestingly members of this protein family have been known to play an important role following infection. In fish however only a limited number of investigations have addressed their role after infection. Being such a diverse group of proteins, more work is needed in order to established and characterized the exact function of these proteins. In this study we therefore wanted to investigate changes in protein abundance of after a mimicked infection in rainbow trout (Oncorhynchus mykiss), using an established model. In the model infection is mimicked by a well-defined tissue damage allowing each fish to be equally affected. Samples were taken 7 days after tissue damage and included samples from the damaged tissue, internal control and an external control. Changes in protein expression between the wound, internal- and external control were found using a t-test. To investigate numerous proteins in a single study changes in protein abundance were investigated using 2-dimensional gel electrophoresis. Protein of interest were identified using MALDI MS/MS. The results show that both annexin 4 and 5 are regulated after tissue damaged on the protein level. These proteins have been assign to functions like regulation of coagulation, apoptosis, and exocytosis, indicating their importance following infection and subsequent repair in fish. In addition the regulation observed in this study are supported by previous findings on the mRNA level, where both proteins are regulated following infection. In conclusion this study show regulation on the protein level of two members of the annexin protein family after infection like tissue damage

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Regulation of annexins following infection like tissue damage – investigated by 2-dimensional gel electrophoresis.

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Regulation of annexins following infection like tissue damage – investigated by 2-dimensional gel electrophoresis.

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