A Ciuffi

A Hofmann

A Onishi

A Wilber

AB Tolstrup

AE Davidson

AJ Dupuy

Anders Lade Nielsen

AR Schroder

Arne Lund Jørgensen

B Moldt

Brian Dall Schyth

Brian Moldt

C Kubota

CB Whitelaw

D Garrick

DA Yergeau

G Liu

Gábor Vajta

H Singh

H Zayed

Henrik Callesen

I Wilmut

IA Polejaeva

Ida E. Holm

J Betthauser

Jacob Giehm Mikkelsen

Jannik E. Jakobsen

JB Cibelli

JG Mikkelsen

JR Ohlfest

Juan Li

K Naruse

KJ Clark

Kjeld Dahl Winther

KW Park

L Lai

L Liu

L Mates

L Sinzelle

Lars Bolund

Lin Lin

M Hirabayashi

M Schorpp

MB Nottle

Mette Schmidt

NH Staunstrup

NL Webster

P Leahy

Peter M. Kragh

PM Kragh

RA Cabot

RE Hammer

RS Mitchell

S Hyun

S Watanabe

SR Yant

T Dalsgaard

T Wakayama

TJ Vigdal

Y Du

Y Kato

Ying Liu

Z Ivics

Z Izsvak

Jakobsen, Jannik E.

Li, Juan

Kragh, Peter Michael

Moldt, Brian

Lin, Lin

Liu, Ying

Schmidt, Mette

Winther, Kjeld Dahl

Schyth, Brian Dall

Holm, Ida E.

Vajta, Gabor

Bolund, Lars

Callesen, Henrik

Jørgensen, Arne Lund

Nielsen, Anders Lade

Mikkelsen, Jacob Giehm

Copenhagen University Research Information System

Pig transgenesis by sleeping beauty DNA transposition

Crossref

Transgenic Research

Pig transgenesis by Sleeping Beauty DNA transposition

Modelling of human disease in genetically engineered pigs provides unique possibilities in biomedical research and in studies of disease intervention. Establishment of methodologies that allow efficient gene insertion by non-viral gene carriers is an important step towards development of new disease models. In this report, we present transgenic pigs created by Sleeping Beauty DNA transposition in primary porcine fibroblasts in combination with somatic cell nuclear transfer by handmade cloning. Göttingen minipigs expressing green fluorescent protein are produced by transgenesis with DNA transposon vectors carrying the transgene driven by the human ubiquitin C promoter. These animals carry multiple copies (from 8 to 13) of the transgene and show systemic transgene expression. Transgene-expressing pigs carry both transposase-catalyzed insertions and at least one copy of randomly inserted plasmid DNA. Our findings illustrate critical issues related to DNA transposon-directed transgenesis, including coincidental plasmid insertion and relatively low Sleeping Beauty transposition activity in porcine fibroblasts, but also provide a platform for future development of porcine disease models using the Sleeping Beauty gene insertion technology

Pig transgenesis by Sleeping Beauty DNA transposition

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Copenhagen University Research Information System

Crossref

Online Research Database In Technology