Jakobsen, Sune

Jonsson, Gunnar Eigil

Korsholm, Lars

Woodley, John

Yuan, Linfeng

Online Research Database In Technology

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Downloaded from orbit.dtu.dk on: Dec 18, 2017Electro-membrane filtrationAn Alternative Way to Fractionate Industrial EnzymesYuan, Linfeng; Korsholm, Lars; Jakobsen, Sune; Woodley, John; Jonsson, Gunnar EigilPublication date:2011Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Yuan, L., Korsholm, L., Jakobsen, S., Woodley, J., & Jonsson, G. E. (2011). Electro-membrane filtration: AnAlternative Way to Fractionate Industrial Enzymes. Poster session presented at ICOM 2011 : InternationalCongress on Membranes and Membrane Processes, Amsterdam, .ResultsInvestigation of operation TMPOperation way and pH selectionFigure 5 Selection of operating wayFigure 6 Selection of solution pHSet-up & PrincipleFigure 2 Experimental Figure 3 Principle ofset-up EMFIntroductionIn the application of industrial enzymes separation,pressure driven-membrane filtration is limited to itslow selectivity.Enevoldsen and co-workers [1,2] have shown that byusing an electric field during crossflow ultrafiltration(EUF) of industrial enzyme solutions, an improvementin flux of 3-7 fold has been obtained.EMF combines conventional membrane filtration withelectrodialysis. In comparison with pressure-drivenmembrane filtration an increase in selectivity for theseparation of charged components can be expected.Figure 1 Model of the operation boundary for EMF and pressure-driven membrane filtrationAcknowledgmentsThe Novozyme Bioprocess Academy is acknowledged for the financial supportof this project.References[1] A.D. Enevoldsen, Electrically enhanced ultrafiltration of industrial enzymesolutions, Technical University of Denmark, Ph.D. thesis, 2007[2] A.D. Enevoldsen, E.B. Hansen and G.Jonsson, Electro-ultrafiltration ofindustrial enzyme solutions, Journal of Membrane science 299(2007) 28-37Conclusion• By applying the electric field, PLA and LP were separated and the selectivity improved ascompared to normal filtration, but the improvement was not impressive. The selectivity was alsodependent on the feed concentration.• Productivity and selectivity were not good, which seemed to be related with the solubility issueof the enzymes dependent on pH and feed conductivity. More work should be done to reduce thefouling and increase the flux• It seemed that LP was more easier to foul the membrane. Therefore, the other operation waywhich PLA is going to be removed from permeate should be tried.Methodology• System operated in a batch-wise manner (refer toFig.2).• Two industrial enzymes: phospholipase (PLA) withMW 13.3KDa, PI 7.68 and lipase (LP) with MW29.3KDa,PI 4.7 were used.• Why PLA and LP were chosen? Pressure-drivenmembrane filtration is not possible to separate themdue to their close MW.Figure 4 Calculated charge property of PLA and LP asfunction of solution pHLinfeng Yuan1, Lars Korsholm2, Sune Jakobsen2 , John Woodley1, Gunnar Jonsson11Department of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark2Recovery Development, Novozymes A/S, Smørmosevej 9, 3AM, DK-2880 Bagsvæ rd, DenmarkElectro-membrane filtration: An Alternative Way to Fractionate Industrial Enzymes,, ,i i ii i convi diff i elecvi iJ J J Jdc dED Fzc c Jdx dx    Figure 7 Filtration of 2g/L LP at solution pH 7.2 and 4.7 (A)flux as function of TMP (B) transmission as function of TMP02040608010030min 60min 90minTransmission (%)+-+++F PE E Figure 8 Effect of electricfield on the transmissionof PLA.Experimentsoperated with initial 15g/Lfeed solution, solution pHat 5 and TMP at 0.4bar(■)MF(□)-MF+Effect of electric fieldSeparation of PLA and LPTable 1 Selectivity and LP purity improvement in EMFExperiment pH Feed concentration (g/L) Percentage of LP concentration in feed(%) Selectivity Permeate LP purity (%)10min 90min 10min 90minMF, 0.35bar 5 21.8 23.2 1.01 0.84 23.34 20.32-MF+,0.35bar,30 V 5 19.1 22.4 2.46 3.49 41.45 50.13Experiment pH Feed concentration (g/L) Percentage of LP concentration in feed(%) Selectivity Permeate LP purity (%)10min 90min 10min 90min-MF+,0.35bar,30 V 5 19.1 22.4 2.46 3.49 41.45 50.13-MF+,0.35bar,30 V 5 10.2 21.7 2.88 5.13 44.42 58.74Table 2 Selectivity and LP purity in permeateobtained at different feed concentration0204060800 0,5 1 1,5 2Flux(LMH)TMP (bar)pH 7.2 step up pH 7.2 step down pH 4.7 step up pH 4.7 step downA010203040500 0,5 1 1,5 2Transmission (%)TMP (bar)pH 7.2 step up pH 7.2 step down pH 4.7 step up pH 4.7 step downB

Electro-membrane filtration:An Alternative Way to Fractionate Industrial Enzymes

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Electro-membrane filtration:An Alternative Way to Fractionate Industrial Enzymes

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