Christensen, Mikkel Black

Einer-Jensen, Katja

Evensen, Øystein

Gautier, Laurent

Lorenzen, Ellen

Lorenzen, Niels

Martin, Samuel

Rasmussen, Jesper Skou

Schyth, Brian Dall

Villanueva, Sara A.

A DNA vaccine encoding the glycoprotein (G) genes of the salmonid rhabdovirus viral haemorrhagic septicaemia virus (VHSV) has proven highly efficient against the disease caused by this virus in rainbow trout (Oncorhynchus mykiss). Several studies have demonstrated that this vaccine induces both an early unspecific antiviral response as well as a long-lasting specific protection. However, temperature appears to influence immune response with respect to the nature and duration of the protective mechanisms. In this study, groups of fish were temperature acclimated, vaccinated and challenged at three different temperatures (5, 10 and 15ºC). Tissue and organ samples were collected at numerous time points post vaccination (pv) and post viral challenge (pch). Then, gene expression levels of a two immune genes (Vig-1 and Mx3) involved in unspecific antiviral response mechanisms were determined by Q-PCR. The expression profiles appeared similar for the two genes in terms of temperature dependency with a faster induction and shorter duration at the higher temperature. In order to analyze the temperature effect on the relative expression profiles across a larger set of immune genes time points displaying similar Mx3 expression levels post vaccination were identified: 4 days pv (15ºC); 7 days pv (10ºC); 23 days pv (5ºC), respectively. A targeted trout immune gene array including probes for VHSV genes was used for analysis of vaccinated vs control fish per temperature (4-5 replicates). Temperature altered the transcriptome response to the viral challenge, and the number of responsive genes increased at higher temperature: 51 (5ºC), 64 (10ºC) and 72 (15ºC), respectively, indicating that fish kept at higher temperature may have an enhanced immune response. Additional correlation analysis allowed identification of genes that were significantly up- or down regulated synchronous with expression of the vaccine G gene. These findings encompass that for example in fish kept at 5ºC , putative CD3, CD4, CD9, CD28, CD53, CD63, CD83, CD84 were up regulated, while CD59, CD83 were down regulated, potentially indicating balancing mechanism of the immune system. An experimental VHSV challenge was performed 7 weeks pv. Similar protection levels of approximately 10% mortality were found for the vaccinated fish, regardless of temperature during immunisation and challenge, whereas the course and level of mortality among the controls were temperature dependent. At day 5 post infection, the number of differentially regulated genes in the livers of vaccinated versus control fish was highest in fish kept at 10ºC and lowest in fish at 5ºC. This difference presumably reflects the temperature dependent progression of the disease in the controls. Further analysis of the obtained data with respect to gene regulation pathways as a result of DNA vaccination and/or viral infection will be presented

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Downloaded from orbit.dtu.dk on: Dec 18, 2017Temperature influences the expression profiling of immune response genes in rainbowtrout following DNA vaccination and VHS virus infectionEiner-Jensen, Katja; Gautier, Laurent; Rasmussen, Jesper Skou; Lorenzen, Ellen; Christensen, MikkelBlack; Villanueva, Sara A.; Martin, Samuel; Evensen, Øystein; Schyth, Brian Dall; Lorenzen, NielsPublication date:2011Link back to DTU OrbitCitation (APA):Einer-Jensen, K., Gautier, L., Rasmussen, J. S., Lorenzen, E., Christensen, M. B., Villanueva, S. A., ...Lorenzen, N. (2011). Temperature influences the expression profiling of immune response genes in rainbowtrout following DNA vaccination and VHS virus infection. Abstract from Joint Western Fish Disease Workshop &AFS fish Health Section meeting, Nanaimo, British Columbia, Canada, .TEMPERATURE INFLUENCES THE EXPRESSION PROFILING OF IMMUNE RESPONSE GENES IN RAINBOW TROUT FOLLOWING DNA VACCINATION AND VHS VIRUS INFECTION Katja Einer-Jensen1, Laurent Gautier2, Jesper S. Rasmussen1, Ellen Lorenzen1, Mikkel B. Christensen1, Sara A. Villanueva3, Samuel Martin4, Uwe Fischer5, Øystein Evensen6, Brian D. Schyth1, Niels Lorenzen1 1 National Veterinary Institute, Technical University of Denmark, Århus, Denmark. 2 The Multi-Assay Core (DMAC), Center for Biological Sequence Analysis, Technical University of Denmark, Denmark. 3Bionostra Biotechnology Applications, S.L.U. Bionostra Group, Spain 4 Scottish Fish Immunology Research Centre, University of Aberdeen, Scotland, United Kingdom 5 Laboratory for Fish Immunology, Friedrich Loeffler Institute, Germany 6 Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, Norway  A DNA vaccine encoding the glycoprotein (G) genes of the salmonid rhabdovirus viral haemorrhagic septicaemia virus (VHSV) has proven highly efficient against the disease caused by this virus in rainbow trout (Oncorhynchus mykiss). Several studies have demonstrated that this vaccine induces both an early unspecific antiviral response as well as a long-lasting specific protection. However, temperature appears to influence immune response with respect to the nature and duration of the protective mechanisms. In this study, groups of fish were temperature acclimated, vaccinated and challenged at three different temperatures (5, 10 and 15ºC). Tissue and organ samples were collected at numerous time points post vaccination (pv) and post viral challenge (pch). Then, gene expression levels of a two immune genes (Vig-1 and Mx3) involved in unspecific antiviral response mechanisms were determined by Q-PCR. The expression profiles appeared similar for the two genes in terms of temperature dependency with a faster induction and shorter duration at the higher temperature. In order to analyze the temperature effect on the relative expression profiles across a larger set of immune genes time points displaying similar Mx3 expression levels post vaccination were identified: 4 days pv (15ºC); 7 days pv (10ºC); 23 days pv (5ºC), respectively.  A targeted trout immune gene array including probes for VHSV genes was used for analysis of vaccinated vs control fish per temperature (4-5 replicates). Temperature altered the transcriptome response to the viral challenge, and the number of responsive genes increased at higher temperature: 51 (5ºC), 64 (10ºC) and 72 (15ºC), respectively, indicating that fish kept at higher temperature may have an enhanced immune response. Additional correlation analysis allowed identification of genes that were significantly up- or down regulated synchronous with expression of the vaccine G gene. These findings encompass that for example in fish kept at 5ºC , putative CD3, CD4, CD9, CD28, CD53, CD63, CD83, CD84 were up regulated, while CD59, CD83 were down regulated, potentially indicating balancing mechanism of the immune system. An experimental VHSV challenge was performed 7 weeks pv. Similar protection levels of approximately 10% mortality were found for the vaccinated fish, regardless of temperature during immunisation and challenge, whereas the course and level of mortality among the controls were temperature dependent. At day 5 post infection, the number of differentially regulated genes in the livers of vaccinated versus control fish was highest in fish kept at 10ºC and lowest in fish at 5ºC. This difference presumably reflects the temperature dependent progression of the disease in the controls. Further analysis of the obtained data with respect to gene regulation pathways as a result of DNA vaccination and/or viral infection will be presented.  This work was supported by the EC FP6 project IMAQUANIM (more info at www.imaquanim.eu) Presented during the 52nd Joint Western Fish Disease Workshop & AFS fish Health Section meeting June 14-16, 2011 Vancouver Island Conference Centre, Nanaimo, British Columbia, Canada 

Temperature influences the expression profiling of immune response genes in rainbow trout following DNA vaccination and VHS virus infection

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Temperature influences the expression profiling of immune response genes in rainbow trout following DNA vaccination and VHS virus infection

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