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Complex electrochemical and impedimetric evaluation of DNA damage by using DNA biosensor based on a carbon screen-printed electrode
Authors
Labuda J.
Ziyatdinova G.
Publication date
1 January 2011
Publisher
Abstract
DNA biosensor (DNA/SWCNT-COOH-CHIT/SPCE) composed of dsDNA adsorptive layer on a carboxylated single-walled carbon nanotubes-chitosan composite deposited at a commercial carbon based screen-printed electrode has been prepared and applied to a complex investigation of damage to DNA by the Fenton type cleavage agent (hydroxyl radicals formed in the mixture of Cu2+, H 2O2 and ascorbic acid) and copper(ii)-quercetin system in 0.1 M PBS pH 7.0 under aerobic conditions. The dsDNA damage detection is performed by using square-wave voltammetry (SWV), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in 1 × 10-7 M thioridazine and 1 × 10-3 M K4[Fe(CN) 6]/K3Fe(CN)6 in the 0.1 M phosphate buffer solution, pH 7.0. Initial enhancement of the intrinsic guanine and adenine moieties SWV response over that of original dsDNA one indicates opening of the helix structure in the first stage of damage. At the same time, decrease in the intercalated thioridazine response confirms damage of the helix structure in parallel to deep degradation of the DNA chain and its removal from the electrode surface as indicated by the CV and EIS measurements in the presence of the [Fe(CN)6]3-/4- redox indicator in solution. © 2011 The Royal Society of Chemistry
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Kazan Federal University Digital Repository
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oai:dspace.kpfu.ru:net/103152
Last time updated on 07/05/2019
Kazan Federal University Digital Repository
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:dspace.kpfu.ru:net/139859
Last time updated on 07/05/2019