Subtilisin-like proteinase secreted by the Bacillus pumilus KMM 62 strain at different growth stages

Abstract

Proteinase secreted in the environment by bacilli on different growth stages was isolated by ion chromatography from the culture medium of Bacillus pumilus KMM 62. According to the hydrolysis character of specific chromogenic substrates and inhibition type, the enzyme belongs to subtilisin-like serine proteinases. The isolated proteinase with the molecular mass of 30 kDa displays maximum activity on hydrolysis of the peptide substrate Z-Ala-Ala-Leu-pNA at pH 8.0 - 8.5 and temperature 30°C. The protein is stable in the range of pH 7.5 - 10.0. It was shown that subtilisin-like serine proteinase from B. pumilus KMM 62 possessed thrombolytic activity. © Pleiades Publishing, Ltd., 2012