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Bi-enzyme sensor based on thick-film carbon electrode modified with electropolymerized tyramine
Authors
Brainina K.
Budnikov H.
Evtugyn G.
Suprun E.
Publication date
1 January 2004
Publisher
Abstract
Bi-enzyme sensor based on thick-film epoxy-carbon electrode modified with polytyramine has been developed and examined for the determination of peroxidase substrates and cholinesterase inhibitors. Polytyramine was obtained on the electrode surface by repeated scanning of the potential from +600 to +1800 mV vs. Ag/AgCl in tyramine solution. The enzymes were immobilized in the polytyramine matrix by cross-linking with glutaraldehyde. The biosensor developed provides a reliable and inexpensive way for preliminary testing of common environmental pollutants with a single sensor in accordance with assumed toxic effect by the choice of appropriate substrate and measurement conditions. The bi-enzyme sensor makes it possible to determine substituted phenols and aromatic amines in the micromolar range of their concentrations and anticholinesterase pesticides with detection limits of 0.1 (Coumaphos) and 0.03 μmol l-1 (Chloropyrifos-methyl). © 2004 Published by Elsevier B.V
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Kazan Federal University Digital Repository
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oai:dspace.kpfu.ru:net/135188
Last time updated on 07/05/2019
Kazan Federal University Digital Repository
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:dspace.kpfu.ru:net/99124
Last time updated on 07/05/2019