Uttenthal, Åse

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Dec 18, 2017Differentiating infection in vaccinated animalsWork Package 4.3 DIVA diagnosticsUttenthal, ÅsePublication date:2011Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Uttenthal, Å. (2011). Differentiating infection in vaccinated animals: Work Package 4.3 DIVA diagnostics. Postersession presented at 5th Annual Meeting EPIZONE, Arnhem, Netherlands.Differentiating infection in vaccinated animalsWork Package 4.3 DIVA diagnosticsTASKS:1. Validation of DIVA diagnostics √2. Development of confirmatory tests √3. Implementation of DIVA diagnostics for existing vaccines √4. Build networks to improve DIVA diagnostics √Serologic DIVA diagnostics:BTV DIVA diagnostics: •The first results obtained indicate that BTV-inactivated vaccines used in the field are not free of NS 2 and 3 proteins. A better purification of these vaccines is necessary to develop this kind of DIVA strategy. Genetic CSFV DIVA diagnostics:Animals vaccinatedwith live vaccines maybecome viremic. Several real-time RT-PCR methods have been published for differentiation of C-strains from Classicalswine fever virus wild-types.How was dissemination?Three workshops on FMD NSP for stakeholders and authorities during 2007Joint international publications on DIVA diagnostics and a review on DIVA diagnosticswww.epizone-eu.netEC Network of Excellence for  epizootic disease diagnosis and controlDIVA vaccines and diagnostics will improve the vaccinate-to-live strategy for domestic livestock as well as free ranging animals. Safe trade of healthy, vaccinated animals.WP leader: Åse Uttenthal, National Veterinary Institute DTU, Lindholm, Denmark. ASUT@VET.DTU.DK•NS1 antibodies are not detected in vaccinated animals.CSFV DIVA diagnostics: •For the evaluation of CSFV Antibody ELISAs seven commercially available test kits from seven providers were tested in five CSF reference laboratories. •New expression systems are being tried for production of antigens for serological tests. •New detection systems for multiplex assays are analysed.•New chimeric pestivirus vaccines are currently validated.FMDV DIVA diagnostics: •Existing ELISA for the detection of antibodies to FMDV NSP test (3ABC-trapping ELISA) has been optimized. This work led to the availability of a ready-to-use, stable kit, then usable also in poorly equipped laboratories.• Confirmatory tests based on IgA in saliva are developed.HPAI DIVA diagnostics:•An inter-laboratory evaluation of a commercial DIVA kit was performed. There was good specificity and fair sensitivity when tested by 8 EPIZONE partners.National publications and presentations on DIVA diagnostics has improved awarenessNational and EU funding for DIVA vaccines and diagnostics has been achievedThanks to all partners for their enthusiam

Differentiating infection in vaccinated animals:Work Package 4.3 DIVA diagnostics

http://orbit.dtu.dk/ws/files/5546292/2011%20AM%20WP%204%203.pdf

Differentiating infection in vaccinated animals:Work Package 4.3 DIVA diagnostics

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