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Amperometric immunosensor for nonylphenol determination based on peroxidase indicating reaction
Authors
Budnikov H.
Eremin S.
+3 more
Evtugyn G.
Ismagilova A.
Shaljamova R.
Publication date
1 January 2006
Publisher
Abstract
Novel immunosensor for nonylphenol (NP) determination has been developed by immobilization of specific antibodies together with horseradish peroxidase on the surface of carbon screen-printed electrode. The signal of the immunosensor is generated by the involvement of NP accumulated in the peroxidase oxidation of mediator (Methylene Blue, hydroquinone or iodide). This results in the increase of the signal recorded by linear-sweep voltammetry. The sensitivity of the detection depends on the nature of mediator, its concentration and incubation period. Cross-selectivity of the response toward readily oxidized phenolic compounds has been determined. The immunosensor developed makes it possible to detect from 20 μg L-1 to 44 mg L-1 of NP with detection limit 10 μg L-1 of NP. © 2005 Elsevier B.V. All rights reserved
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Kazan Federal University Digital Repository
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oai:dspace.kpfu.ru:net/96904
Last time updated on 07/05/2019
Kazan Federal University Digital Repository
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:dspace.kpfu.ru:net/137411
Last time updated on 07/05/2019