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Selenoprotein P as a diabetes-associated hepatokine that impairs angiogenesis by inducing VEGF resistance in vascular endothelial cells
Authors
Ando Hitoshi
Chikamoto Keita
+22 more
Fujimura Akio
Ishikura Kazuhide
Kameo Satomi
Kaneko Shuichi
Kato Kenichiro
Kidoya Hiroyasu
Kumazaki Masafumi
Lan Fei
Matsuzawa-Nagata Naoto
Misu Hirofumi
Miyamoto Ken-ichi
Okamoto Yasuo
Ota Tsuguhito
Saito Yoshiro
Sakurai Masaru
Tajima Natsumi
Takahashi Kazuhiko
Takakura Nobuyuki
Takamaura Toshinari
Takayama Hiroaki
Takeshita Yumie
Takuwa Yoh
Publication date
1 September 2014
Publisher
'Springer Science and Business Media LLC'
Doi
Cite
Abstract
Aims/hypothesis Impaired angiogenesis induced by vascular endothelial growth factor (VEGF) resistance is a hallmark of vascular complications in type 2 diabetes; however, its molecular mechanism is not fully understood. We have previously identified selenoprotein P (SeP, encoded by the SEPP1 gene in humans) as a liver-derived secretory protein that induces insulin resistance. Levels of serum SeP and hepatic expression of SEPP1 are elevated in type 2 diabetes. Here, we investigated the effects of SeP on VEGF signalling and angiogenesis. Methods We assessed the action of glucose on Sepp1 expression in cultured hepatocytes. We examined the actions of SeP on VEGF signalling and VEGF-induced angiogenesis in HUVECs. We assessed wound healing in mice with hepatic SeP overexpression or SeP deletion. The blood flow recovery after ischaemia was also examined by using hindlimb ischaemia model with Sepp1-heterozygous-knockout mice. Results Treatment with glucose increased gene expression and transcriptional activity for Sepp1 in H4IIEC hepatocytes. Physiological concentrations of SeP inhibited VEGF-stimulated cell proliferation, tubule formation and migration in HUVECs. SeP suppressed VEGF-induced reactive oxygen species (ROS) generation and phosphorylation of VEGF receptor 2 (VEGFR2) and extracellular signal-regulated kinase 1/2 (ERK1/2) in HUVECs. Wound closure was impaired in the mice overexpressing Sepp1, whereas it was improved in SeP-/-mice. SeP+/-mice showed an increase in blood flow recovery and vascular endothelial cells after hindlimb ischaemia. Conclusions/interpretation The hepatokine SeP may be a novel therapeutic target for impaired angiogenesis in type 2 diabetes. © 2014 Springer-Verlag Berlin Heidelberg
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Last time updated on 06/05/2019