A Cornish-Bowden

AM Fordyce

AR Neves

B Poolman

BE Terzaghi

BJ Koebmann

BM Bakker

C Garrigues

C Platteeuw

C Solem

DG Fraenkel

EJ Luesink

FL Felipe

H Israelsen

H Kacser

HP Smits

HW Andersen

I Schaaff

J Hugenholtz

J Thompson

JA Wouters

JL Snoep

JS Hofmeyr

L Brpndsted

M Cocaign-Bousquet

M Kleerebezem

MHN Hoefnagel

MJ Gasson

NB Jensen

PG Ruyter

PR Jensen

PW Mason

R Heinrich

RM Llanos

S Thomas

TD Thomas

VL Crow

Journal of Insect Science

English

PubMed

The understanding of control of metabolic processes requires quantitative studies of the importance of the different enzymatic steps for the magnitude of metabolic fluxes and metabolite concentrations. An important element in such studies is the modulation of enzyme activities in small steps above and below the wild-type level. We review a genetic approach that is well suited for both Metabolic Optimization and Metabolic Control Analysis and studies on the importance of a number of glycolytic enzymes for metabolic fluxes in Lactococcus lactis. The glycolytic enzymes phosphofructokinase (PFK), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate kinase (PYK) and lactate dehydrogenase (LDH) are shown to have no significant control on the glycolytic flux in exponentially growing cells of L. lactis MG1363. Introduction of an uncoupled ATPase activity results in uncoupling of glycolysis from biomass production. With MG1363 growing in defined medium supplemented with glucose, the ATP demanding processes do not have a significant control on the glycolytic flux; it appears that glycolysis is running at maximal rate. It is likely that the flux control is distributed over many enzymes in L. lactis, but it cannot yet be excluded that one of the remaining glycolytic steps is a rate-limiting step for the glycolytic flux

Købmann, Brian Jensen

Andersen, Heidi Winterberg

Solem, Christian

Jensen, Peter Ruhdal

Online Research Database In Technology

Experimental determination of control of glycolysis in Lactococcus lactis

James S. Miller

Lee Dyer

Crossref