\u3cp\u3eRecent advances in transmission electron microscopy (TEM) have led to unprecedented automated control over data acquisition for various applications. Improvement of methods for obtaining and reconstructing tilt series projections allow us to create three-dimensional tomographic representations of bulk materials at the nanoscale. We demonstrate the combination of the latest TEM technology and tomography, at cryogenic temperatures, for the observation of soft matter such as polymers, hybrid coatings, engineered proteins and nanoparticles for targeted gene and drug delivery, nanocomposites and biomaterials, in conjunction with stateof-the-art vitrification methods for specimen preparation. Using such advanced Cryo-TEM, we aim to understand the organization of molecules, macromolecules and biomolecules, in order to control the properties and performance of materials and devices.\u3c/p\u3
