We describe for the first time the advertisement call of Pristimantis subsigillatus from southern Ecuador. Our study provides a detailed quantitative characterization of the advertisement call of P. subsigillatus, filling a gap in our knowledge of this genus, the most speciose among vertebrates. Males called perched on vegetation 0.5-2.5 m above ground, always during mild rain. The advertisement call is composed of a single note with a duration of 63-80 ms, with an initial short pulse (3-10 ms) followed by a longer tonal component. Call rates ranged between 4-12 calls/min. The dominant frequency varied between 2.02-2.82 kHz.The Secretaría de Educación Superior, Ciencia, Tecnología e Innovación, Republic of Ecuador (SENESCYT) provided funding for Paul Székely and Dan Cogălniceanu through the Prometeo Project. The Jocotoco Foundation provided access to Buenaventura Forest Reserve. Florina Stănescu, Paul Székely and Dan Cogălniceanu received additional support from the SYNTHESYS Project. The SYNTHESYS Project is financed by the European–Community–Research Infrastructure Action under the FP7 “Capacities” Specific Programme. Partial call analyses and call deposit were funded by project TATANKA (CGL2011-25062), Ministerio de Ciencia e Innovación, FCW (CGL2011-16159-E). We are grateful to Dr. Santiago Ron, Pontificia Universidad Católica de Ecuador for his support. Collecting permits were granted to Pontificia Universidad Católica de Ecuador as No. 005-14 IC-FAUDNB/MA and No. 003-15 IC-FAU-DNB/MA.Peer reviewe
