Assessing the sustainability of Litopenaus vannamei on white spot virus vaccine and fed with yeast Sacchromayces cerevisiae and algae Gracilaria corticata in the face of white spot syndrome virus

Abstract

Efficacy of G. corticata, S. cerevisiae and gamma irradiation WSSV as immunostimulants to white shrimp L. vannamei was investigated. 1200 healthy shrimp SPF L. vannamei subadult with average weight of 10±1.02 g were collected and divided to four groups. The first group (T1) fed with commercial pellet, second group (T2) fed S. cerevisiae (2 g/ Kg), third group (T3) fed G. corticata powder was mixed with shrimp feed (2 g/Kg) and fourth group (T4) fed commercial pellet and injected intramuscularly gamma irradiant WSSV (1µl/gbw). The shrimp were fed on these diets for a period of 10 days and then challenged injected with WSSV and maintained for 25 days. The control group maintained same manner but without inject with WSSV. Survival rate and immune parameters such as total hemocyte count (THC), total protein plasma (TPP), superoxide dismutase (SOD) activity, peroxidase (POD) activity and phenoloxidas activity (PO) were determined. The result indicated survival rate in shrimp group T4 was 57.05±3.52%, shrimp group T3 was 22.5±0.5% and shrimp group T2 was 15±1.05% and shrimp group T1 was 00.0±0%, respectively. The shrimp group T4 showed higher hematological data, i.e. THC, TPP, SOD, POD and PO at the end of experiment. This study showed that gamma irradiant WSSV are effective immunostimulants in shrimp L. vannamei and perform better than the G. corticata and S. cerevisiae