Residual level of 17- Beta Stradiol and progesterone hormones in rainbow trout fish plasma were measured during different period using RIA method. Blood sampling from abdominal aorta were taken from 70 individual of female fishes (100±11 g) which had been exposed to hormone at 0.5, 1,2,4,8,12,24 and 168 h (7 groups) compared with control group which was not exposed to this hormone. Results showed that plasma hormones measurement in different fish groups after exposing had significant differences (P<0.01) and the highest and lowest 17-Beta Stradiol hormone residue were observed in fishes that exposed 0.5h and 168h to hormone respectively (121±9 ng. ml^-1 and 3±0.9 ng. ml^-1) but there is no any differences between fishes exposed 168h to hormone and control group. Also the highest progesterone hormone level were measured in fishes 0.5 an 1h exposed and the lowest one was in fishes 168 h exposed. The range of progesterone hormone were between 0.3 to 1.1 ng .ml^-1 and significant increasing of this hormone levels were obtained in fishes exposed to hormone 4 to 24h (P<0.01). As consequence these hormone can not residue in fishes for a long time and maximum after one week the levels back to the normal
