The presnt study has been carried out to evaluate the Nannochloropsis oculata culture performance in 100L columnar plastic bags under two chemical media (TMRL and CONWAY) and 3 light regimes in the controlled conditions. Algae used for the experiment has been provided through culture of primitive stock and by transferring it to 2 L glassy erlenmeyer and 15 L plastic containers (as intermediate stage) using CONWAY media respectively. Daily evaluation of algae concentration was carried out during the 18 days culture period. At the end of the study produced algae has been sedimented to be used for evaluation of the dried matter and algae biomass. Results of the study showed that the concentration of the algae culture was 22±3.34×106 cells. ml^-1, at 8th day, and reached the concentration of 26.58±4.02×106 cells. ml^-1 at the final day of the culture course. Although the results revealed the possibility of achievement of acceptable concentration for the N.oculta by the method adopted in the present study, but the achievement was not such favourable to be considered as an altenative for the common method. Nevertheless it could be considered as an alternative for the constrained situations. Moreover according to the results there was no significant algae concentration difference between light regimes (P>0.05). Similar results were observed for the interaction effect of chemical media and light regimes. Meanwhile the ratio of dried matter obtained from algae under the TMRL media was significantly (P0.05) affect the algae production. Finally it could be concluded that the N.oculata algae could be successfully cultured in plastic columnar bags under the controlled conditions but the method adopted in the present study could be applicable in the improper conditions
