Complementary feedstuff extract from Laminaria digitata and Ascophylum nodosum that containing 1% alginic acids as shrimps stimulating of immune system for control of WSSV is used in this study. The test shrimps Litopenaeus vannamei in larvae stage (Z1-PL1), post larvae stage (PL1-PL10) and juvenile (from 30th day to 40th day) fed by complimentary feedstuff comprised control group. The other condition in test and control group was the same manner. Both groups after 40 days exposed to WSSV by oral inoculation. The clinical sign and mortality recorded for 10 days. The results showed the survival rate of exposed group was higher than the control group and it was significant (p<0/05). The result also show the mortality observed in exposed group 48 hours later than the control group. This study can led us to new methods for control of White spot disease

Dashtian Nasab, Aghil

Farsi, O.

Ghaednia, B.

Haghighi, A.

Keshtkar, I.

Mehrabi, M.M.

Mirbakhsh, M.

Sharifi, H.

Yeganeh, V.

Aquatic Commons

          
(WSD)
Litopenaeus vannamei
                     
(WSD)Litopenaeus vannamei 
     
MINISTRY OF JIHAD - E - AGRICULTURE 
AGRICULTURAL RESEARCH, EDUCATION & EXTENTION ORGANIZATION 
IRANIAN FISHERIES RESEARCH ORGANIZATION- Iran Shrimp Research Center        
Title:  
Investigation on Effects  of ergosan and vibromax  in 
control of White spot disease(WSD) and Vibriosis in 
shrimp Litopenaeus vannamei       
Executor :  
Aghil Dashtian Nasab        
                                                                                                                                                     
Registration Number 
2009.1214       
                         
Ministry of Jihad  e  Agriculture  
AGRICULTURAL RESEARCH, EDUCATION & EXTENTION ORGANIZATION 
IRANIAN  FISHERIES  RESEARCH  ORGANIZATION    Iran Shrimp Research Center   
Title : Investigation on Effects  of ergosan and vibromax  in control of White spot 
disease(WSD) and Vibriosis in shrimp Litopenaeus vannamei 
Apprpved Number: 4-80-12-88006 
Author:  Aghil Dashtian Nasab 
Executor : Aghil Dashtian Nasab 
Collaborator:B.Ghaednia,M.M,Mehrabi,M.Mirbakhsh,V.Yeganeh,I.Keshtkar, 
O.farsi,H.Sharifi,A.Haghighi  
Advisor(s) : M.Sharifrohani,M.Afsharnasab  
Location of execution : Bushehr province 
Date of Beginning : 2009 
Period of execution : 5 Months 
Publisher : Iranian Fisheries Research Organization 
Circulation : 15 
Date of publishing : 2009 
All Right Reserved . No Part of this Publication May be Reproduced or Reference
    
(WSD)Litopenaeus vannamei
(WSD) Litopenaeus vannamei 
                                                 
Laminaria digitata & Ascophylum nodossum  
 
(V.anguillarum1,2,V.parahemolyticus,V.harvey ,V.vulnificus) 
((WSV Z1-PL1  
PL1 PL10 
. 
) 
WSV .
(P<0.05)
(P<0.05)
 (Rosenbery, 2003)
(Lightner, 2003)
(WSSV)
(Wang et al.,1999; Tapay et al.,1999) 
(Jimenez et al.,2000)
(Lightner,1996; chang et al.,1998;Nunan et al.,1998; Soto et al.,2001; 
Rajendran et al.,1999; Hossain et al.,2001)  (Chang et al.,1998; ; 
Rajendran et al.,1999;Chen et al.2000;Hameed et al.,2003)  (Rajendran et al.,1999; Huang et al.,2001)
DNA
(Van Hulten,2001) 
DNA (Wispoviridae)
.(Van Hulten and vlak, 2001)
(Soto et al.,2001)(Perez et al.,2005) 
1502001-2 
 
1992
(Boyad and Clay 98)
(Skjermo & Vadstein 1999)
.(Aguirre & Ascenico 2000) 
(Lightner 1996)
Mursopenaeus japonicus
 Vibrio flavialis
Penaeus monodonV.harveyiiV.splendidus
(Lavilla et al.,1990)
.( Dierberg and Kiatisimkul ,1996)
(Rosenberry ,96)
(Hopkins & Villalon 1992)
(Itami et al.,1992)
(Raa et al., 1992; Sung et al.,1994; Itami et al.,1996)
(SPF)  (SPR) (Pruder et al.,1995) 
 (Vidal et 
al.,2001; Guan et al.,2003; Jiravanichpaical et al.,2004) 
(Wittveldt et al.,2004)
(Kim et al.,2004)
Penaeus japonicus(Itami et al.,1998; Takashi et al.,2000)
Penaeus monodon(Song et al., 1997)
 (Curdlan) 
1,3
(zymosan) 
MDP 
(Laminaria digitata and Ascophylum nodossum)
Tumor Necrosis Factor alfa(TNF-a)(IL-8)(Peddie et al.,2002) 
 (RTFS)
.(Anon , undated)
Seebass .(Bagni et al., 2004)
Litopenaeus vannamei
 .( Montero et al.,2005)
Laminaria digitata, 
Ascophyllum nodossum  
V.parahemolytius  ,V.vulnifcus, V.harvey, 
V.anguillarum I,II  
IPL12
(WSSV)
(SPF)Liopenaeues vanammei
 
35ppt
Artemia franciscanaINVE
 ppt pH
(1)2 34
Z1-PL1 Z1-PL1 Z1-PL1 Z1-PL1 
M1-PL1M1-PL1M1-PL1M1-PL1
-Z1-PL11-Z1-PL11
PL1-PL12PL1-PL12PL1-PL12PL1-PL12
--PL1-PL10PL1-PL10
Z
M
PL
(1)          
(2)
(3)
(4)
 1 N6 0
2Z1 0/03
3Z20/03
4Z30/03 
5Z3/M10/06
6M10/06
7M20/06
8M30/06
9PL10/08
10PL20/08
11PL30/08
12PL40/01
13PL50/01
14PL60/01
15PL70/12
16PL80/12
17PL90/15
18PL100/15
19PL110/15
(Bio-encapsulation)
II
 Instar II
25
 PL1-PL10
 
One way ANOVATukey Honest Significant Difference (HSD)P<0.05
Litopenaeus vannamei
A
B
(Vibrio harveyii) 
 Z1-PL1PL1 PL10 PL12
A 
B
(Challenge)
TSA
(Lee et al.,1999)
PL52(A , B)
(A1,A2,B1,B2) 
CFU/ml3×10-9
Vibrio harveyii 
A1 +_
A2 ++
B1 __
B2_+
 
One way ANOVA
(HSD)
 
Tukey Honest Significant DifferenceP<0.05
Litopenaeus vannamei
A
B
(WSSV) 
 Z1-PL1PL1 PL10 PL12
A 
B
Fenneropenaeus indicus
(WSD)PCR
WSSV(Challenge)(Prez et al.,2005) 
PL52(A , B)
(A1,A2,B1,B2) 
pH
IHHNV,TSV,WSSV
PCR
A1B1(0.5gr/1shrimp) A2
B2(0.5gr/1shrimp)(Wang et al.,1998)
 WSSV 
A1 +_
A2 ++
B1 __
B2_+
One way ANOVA
(HSD)
 
Tukey Honest Significant DifferenceP<0.05
 0.00%
10.00%
20.00%
30.00%
40.00%
50.00%
60.00%
% 
0
0.001
0.002
0.003
0.004
0.005
0.006
0.007
0.008
0.009
0.01
.
(P<0.01
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%
1 2 3 4 5 6 7 8 9 10
%
 
A1B1
 
A2B2
A2
(P<0.05)
A1 
A2
B1
B2
A1B1A2B2
(P<0.01)
 
 
0 %
1 0 %
2 0 %
3 0 %
4 0 %
5 0 %
6 0 %
7 0 %
8 0 %
9 0 %
1 0 0 %
1 3 5 7 9 1 1 1 3
WSSV
% C u m u la t iv e _ m o r ta l i ty C o n t ro l
% C u m u la t iv e m o r ta l i ty E r g + V ib .
A1B1 A2B2
A2
(P<0.05)
 
A1 
A2
B1
B2 
A1B1A2B2
(P<0.01)
WSSV
 Litopenaeus vannamei
WSSV
Bifidobacterium termophylum
Marsupenaeus japonicusWSSV(Itami et. al.,1998)
CidofovirLitopenaeus vannameiWSSV
(Rahman et al.,2005) 
(Montero et al.,2005)
(WSD)
(Biosecurity)
PCR(Nested PCR)WSSV
V.penaeicida
 
(Sung et al., 1994)
(Itami et al., 1994)
(WSV)
(WSD)
(Witteveldt 2006)
 
 (WSD)

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Wageningen University. ISBN: 90-8504-331-X              
Abstract:  
Complementary feedstuff extract from Laminaria digitata and Ascophylum nodosum that containing 1% alginic 
acids as shrimps stimulating of immune system for control of WSSV is used in this study. The test shrimps 
Litopenaeus vannamei in larvae stage (Z1-PL1), post larvae stage (PL1-PL10) and juvenile (from 30th day to 40th 
day) fed by complimentary feedstuff comprised control group.  The other condition in test and control group was 
the same manner. Both groups after 40 days exposed to WSSV by oral inoculation. The clinical sign and 
mortality recorded for 10 days. The results showed the survival rate of exposed group was higher than the 
control group and it was significant (p<0/05). The result also show the mortality observed in exposed group 48 
hours later than the control group. This study can led us to new methods for control of White spot disease. 
Key words: See weed, Laminaria digitata, Aschophylum nodossum, Litopenaeus vannamei, Control, White spot 
disease  
  


Investigation on effects of ergosan^TM and vibromax^TM in control of white spot disease (WSD) and Vibriosis in shrimp Litopenaeus vannamei

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Investigation on effects of ergosan^TM and vibromax^TM in control of white spot disease (WSD) and Vibriosis in shrimp Litopenaeus vannamei

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