Human pluripotent stem cells (hPSC) are investigated as a source of authentic human cardiac cells for drug discovery and toxicological tests. Cell-based assays performed using an automated fluorescence imaging platform and high-content analysis are valuable in characterizing hypertrophic states that may be induced in hPSC-derived cardiomyocytes upon exposure to cardiotoxic compounds. In high-purity populations of hPSC-derived cardiomyocytes loaded with cell tracer probes and other cell markers, detailed hypertrophic profiles can be assessed based on information captured at cellular and subcellular levels
