AF de Longevialle

AL Chateigner-Boutin

C Lurin

C Schmitz-Linneweber

CC Tseng

D Leister

D Sosso

DA Cushing

DI Arnon

Dongzhi Lin

E Delannoy

E Kotera

F Tavares-Carreón

G De Santis-Maciossek

H Sugimoto

H Yamazaki

Hua Zhou

ID Small

J Hiratsuka

J Kyozuka

J Pfalz

JE Mullet

Jianlong Xu

K Kusumi

K Meierhoff

K Okuda

Kailun Zheng

KJ Livak

KM Gothandam

M Hattori

M Ichinose

MR Karpenahalli

N O’Toole

N Su

PM Williams

PT Hajdukiewicz

QB Yu

QQ Yin

Quan Jiang

R Takeuchi

SA Goff

Sheng Teng

SR Kim

SR McCouch

T Asano

W Cai

W Zhou

X Gong

Xiaodi Gong

Y Hiei

Yanjun Dong

Z Wang

Abstract
          
            Background
            Pentatricopeptide repeat (PPR) proteins play essential roles in modulating the expression of organelle genes and have expanded greatly in higher plants. However, molecular mechanisms of most rice PPR genes remain unclear.
          
          
            Results
            In this study, a new rice PPR mutant, asl3 (albino
              seedling
              lethality3) exhibits an albino lethal phenotype at the seedling stage. This albino phenotype was associated with altered photosynthetic-pigment and chloroplast development. Map-based cloning showed that ASL3 encodes a novel rice PPR protein with 10 tandem PPR motifs, which localizes to the chloroplast. ASL3 showed tissue-specific expression, as it was highly expressed in the chlorenchyma, but expressed at much lower levels in roots and panicles. RNAi of ASL3 confirmed that ASL3 plays an essential role in the early development and chloroplast development in rice. Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant. These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.
          
          
            Conclusions
            The ASL3 gene encoded a novel chloroplast-targeted PPR protein with 10 tandem PPR motifs in rice. Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.
          </jats:sec

Crossref

Rice

The rice ALS3 encoding a novel pentatricopeptide repeat protein is required for chloroplast development and seedling growth

https://thericejournal.springeropen.com/track/pdf/10.1186/s12284-015-0050-9?site=thericejournal.springeropen.com

The rice ALS3 encoding a novel pentatricopeptide repeat protein is required for chloroplast development and seedling growth

Abstract

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