Bonora E

Bradford MM

Bruss MD

Chen WS

Cho H

Christ-Roberts CY

Da Silva AS

Deshmukh A

Du K

Frosig C

Gonzalez E

Hawley JA

Kido Y

Luciano E

Marinho R

Matos A

Oliveira AG

O’Gorman DJ

Patti ME

Pauli JR

Peres SB

Pádua MF

Ropelle ER

Sakamoto K

Tschopp O

Zierath JR

Einstein

 Objective : To investigate the effects of different intensities of acute exercise on insulin sensitivity and protein kinase B/Akt activity in skeletal muscle of obese mice.  Methods : Swiss mice were randomly divided into four groups, and fed either a standard diet (control group) or high fat diet (obese sedentary group and obese exercise group 1 and 2) for 12 weeks. Two different exercise protocols were used: swimming for 1 hour with or without an overload of 5% body weight. The insulin tolerance test was performed to estimate whole-body sensitivity. Western blot technique was used to determine protein levels of protein kinase B/Akt and phosphorylation by protein Kinase B/Akt in mice skeletal muscle.  Results : A single bout of exercise inhibited the high fat diet-induced insulin resistance. There was increase in phosphorylation by protein kinase B/Akt serine, improve in insulin signaling and reduce of fasting glucose in mice that swam for 1 hour without overload and mice that swan for 1 hour with overload of 5%. However, no significant differences were seen between exercised groups.  Conclusion : Regardless of intensity, aerobic exercise was able to improve insulin sensitivity and phosphorylation by protein kinase B/Ak, and proved to be a good form of treatment and prevention of type 2 diabetes.</jats:p

Rodolfo Marinho

Leandro Pereira de Moura

Bárbara de Almeida Rodrigues

Luciana Santos Souza Pauli

Adelino Sanchez Ramos da Silva

Eloize Cristina Chiarreotto Ropelle

Claudio Teodoro de Souza

Dennys Esper Corrêa Cintra

Eduardo Rochete Ropelle

José Rodrigo Pauli

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Einstein (São Paulo)

Effects of different intensities of physical exercise on insulin sensitivity and protein kinase B/Akt activity in skeletal muscle of obese mice

Effects of different intensities of physical exercise on insulin sensitivity and protein kinase B/Akt activity in skeletal muscle of obese mice

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