Baudoin, Jean-Pierre

Bertin, P.

Dje, Yao

Malice, Marie

Tahi, G. C.

Zoro Bi, Arsène I.

We applied a molecular approach using inter-simple sequence repeat (ISSR) markers on three African edible-seeded cucurbits (Citrullus lanatus L. Matsumura and Nakai, Cucumeropsis mannii L. Naudin and Cucumis melo var. agrestis L. Naudin). To obtain clear and reproducible bands on 1.5% agarose gels, we screened 21 ISSR primers and three parameters ( annealing temperature, gel tray, and voltage and running time). The resolution of 11 ISSR markers was performed, with optimal annealing temperature (Ta) varying from 50 to 52 degrees C. The best combination to obtain clear and well-distinguished band patterns was 1.5% agarose gel with a 20-lanes tray (6 mm width) at 80 V for 5 h. Applying the 11 ISSR primers on DNA extracted from an accession of C. lanatus, 66 bands with 4 to 11 bands per primer was observed

Dje, Y.

Tahi, GG

Zoro, BIA

Malice, M

Baudoin, JP

Bertin, Pierre

DIAL UCLouvain

Optimization of ISSR marker for African edible-seeded Cucurbitaceae species' genetic diversity analysis

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Optimization Of Issr Marker For African Edible-Seeded Cucurbitaceae Species' Genetic Diversity Analysis

http://orbi.ulg.ac.be/bitstream/2268/34206/1/2006%20Dje%2c%20Tahi%2c%20Zoro%20Bi%2c%20Malice%2c%20Baudoin%2c%20Bertin.pdf

Optimization Of Issr Marker For African Edible-Seeded Cucurbitaceae Species' Genetic Diversity Analysis

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DIAL UCLouvain

Open Repository and Bibliography - Liège