A Densitometric Method for the Determination of Three Clinically Important Monosaccharides in Urine

Abstract

peer reviewedA quantitative, densitometric, thin layer chromatographic method has been developed for the determination of three clinically important monosaccharides in ureine, after desalting.The analysis of these monosaccharides in urine allows to observe the potential changes induced by antiinflammatories in human small intestine permeability. Xylose, 3-O-methylglucose and rhamnose were estimated quantitatively in urine; arabinose was used as internal standard. The densitometry was performed at a wavelenght of 400 nm after a visualization reaction on HPTLC plates. In all cases, coefficients of variation of the method were less than 2.7%