A Behfar

A Larochelle

A Nevoie

A Valster

A Yúfera

A Zepeda-Moreno

AJ Pelletier

AL Ponte

B Assmus

C Heeschen

C Mothersill

C Urbich

CA Bravery

CR Keese

DE Wright

DF Stroncek

DJ Ceradini

E Chavakis

E Primiceri

EK Frow

EK Sackmann

GP Fadini

H Zheng

H-C Chen

I Bozdag-Turan

II Katkov

J Basu

J Cassidy

J Hesselgesser

J O’Brien

JK Waselenko

JL Lo Surdo

JT Butler

K Guthrie

K Mascotti

M Kucia

M Vasa

MJ Li

MM Rosenkilde

MS Penn

N Dainiak

N Kramer

N Okumura

OI Eseonu

P Ertl

P Jain

P Mauch

P Salmikangas

PM Kern

R Möhle

R Wunderlich

RG Turan

RK Schmidt-Ullrich

RW Franz

S Hatse

S Okabe

S Prokoph

SA Altman

SA Eccles

SY Heazlewood

V Schachinger

W Tan

X Liu

Y Sekine

Y Vagima

AbstractRegenerative medicine studies using autologous bone marrow mononuclear cells (BM-MNCs) have shown improved clinical outcomes that correlate to in vitro BM-MNC invasive capacity. The current Boyden-chamber assay for testing invasive capacity is labor-intensive, provides only a single time point, and takes 36 hours to collect data and results, which is not practical from a clinical cell delivery perspective. To develop a rapid, sensitive and reproducible invasion assay, we employed Electric Cell-substrate Impedance Sensing (ECIS) technology. Chemokine-directed BM-MNC cell invasion across a Matrigel-coated Transwell filter was measurable within minutes using the ECIS system we developed. This ECIS-Transwell chamber system provides a rapid and sensitive test of stem and progenitor cell invasive capacity for evaluation of stem cell functionality to provide timely clinical data for selection of patients likely to realize clinical benefit in regenerative medicine treatments. This device could also supply robust unambiguous, reproducible and cost effective data as a potency assay for cell product release and regulatory strategies.</jats:p

Michael J. Rutten

Bryan Laraway

Cynthia R. Gregory

Hua Xie

Christian Renken

Charles Keese

Kenton W. Gregory

Crossref

Stem Cell Research & Therapy

Rapid assay of stem cell functionality and potency using electric cell-substrate impedance sensing

https://stemcellres.biomedcentral.com/track/pdf/10.1186/s13287-015-0182-2?site=stemcellres.biomedcentral.com

Rapid assay of stem cell functionality and potency using electric cell-substrate impedance sensing

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